Bactérias isoladas de sedimento de manguezal com potencial de biorremediação de contaminações causadas por produtos derivados de petróleo

Abstract

Bioprospecting is search for organisms or their products for biotechnological purposes, and search for microorganisms for contaminants bioremediation has gained prominence. Thus, this study aimed to evaluate the potential for degradation of oil-derived hydrocarbons by 17 bacterial isolates obtained from mangrove sediment. First was performed Gram staining and biochemical tests, analyzed in the IDENTAX software in order to identify the isolates. Then, using petroleum derived products (PDP) as a carbon source, the degradation capacity of these substances by the isolates was evaluated. In Gram stain and colony morphology, the isolates were characterized as gram-positive cocobacilli, with the exception of 4 that showed gram-labile characteristics. It was also possible to observe the presence of artifacts (accumulation of substance and possible production of exopolysaccharides or polymeric substances). The analysis of biochemical tests resulted in the determination of only the genera of the isolates. The PDPdegradation test resulted in three isolates having better degradation capacity, as they act on more than one product. Based on reports in the literature and with the results obtained, it is concluded that bacteria with potential for bioremediation of oil and / or derivatives contamination were obtained, and an indication of selective enrichment effectiveness for bioprospecting microorganisms with biotechnological importance and bioremediation was also obtained.