Immunoinformatic construction of an adenovirus-based modular vaccine platform and its application in the design of a SARS-CoV-2 vaccine
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2020
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The current SARS-CoV-2 pandemic has imposed new challenges and demands for health systems, especially in
the development of new vaccine strategies. Vaccines for many pathogens were developed based on the display of
foreign epitopes in the variable regions of the human adenovirus (HAdV) major capsid proteins (hexon, penton
and fiber). The humoral immune response against the HAdV major capsid proteins was demonstrated to play a
role in the development of an immune response against the epitopes in display. Through the immunoinformatic
profiling of the major capsid proteins of HAdVs from different species, we developed a modular concept that can
be used in the development of vaccines based on HAdV vectors. Our data suggests that different im munomodulatory potentials can be observed in the conserved regions, present in the hexon and penton proteins,
from different species. Using this modular approach, we developed a HAdV-5 based vaccine strategy for SARS CoV-2, constructed through the display of SARS-CoV-2 epitopes indicated by our prediction analysis as im munologically relevant. The sequences of the HAdV vector major capsid proteins were also edited to enhance the
IFN-gamma induction and antigen presenting cells activation. This is the first study proposing a modular HAdV
platform developed to aid the design of new vaccines by inducing an immune response more suited for the
epitopes in display.
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Immunoinformatics, SARS-CoV-2 vaccine, Epitope display, HAdV vectors, Immunomodulatory prediction, Vaccine strategy
Citação
PORTO, Pedro Soares et al. Immunoinformatic construction of an adenovirus-based modular vaccine platform and its application in the design of a SARS-CoV-2 vaccine. Infection Genetics and Evolution, Amsterdam, v. 85, e104489, 2020. DOI: 10.1016/j.meegid.2020.104489. Disponível em: https://www.sciencedirect.com/science/article/pii/S1567134820303208?via%3Dihub. Acesso em: 17 abr. 2025.