Zebrafish (Danio rerio) using as model for genotoxicity and DNA repair assessments: historical review, current status and trends

Abstract

Genotoxic pollutants lead to both DNA damage and changes in cell repair mechanisms. Selecting suitable biomonitors is a fundamental step in genotoxicity studies. Thus, zebrafish have become a popular model used to assess the genotoxicity of different pollutants in recent years. They have orthologous genes with humans and hold almost all genes involved in different repair pathways. Therefore, the aim of the current study is to summarize the existing literature on zebrafish using as model system to assess the genotoxicity of different pollutants. Revised data have shown that comet assay is the main technique adopted in these studies. However, it is necessary standardizing the technique applied to zebrafish in order to enable better result interpretation and comparisons. Overall, pollutants lead to single-strand breaks (SSB), double-strand breaks (DSB), adduct formation, as well as to changes in the expression of genes involved in repair mechanisms. Although analyzing repair mechanisms is essential to better understand the genotoxic effects caused by pollutants, few studies have analyzed repair capacity. The current review reinforces the need of conducting further studies on the role played by repair pathways in zebrafish subjected to DNA damage. Revised data have shown that zebrafish are a suitable model to assess pollutant-induced genotoxicity.