Immunologically reactive M. leprae antigens with relevance to diagnosis and vaccine development

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dc.creatorSampaio, Lucas Henrique Ferreira
dc.creatorStefani, Mariane Martins de Araújo
dc.creatorOliveira, Regiane Morillas
dc.creatorSousa, Ana Lúcia Osório Maroccolo de
dc.creatorIreton, Greg C.
dc.creatorReed, Steven G.
dc.creatorDuthie, Malcolm S.
dc.date.accessioned2019-02-11T11:49:07Z
dc.date.available2019-02-11T11:49:07Z
dc.date.issued2011
dc.description.abstractBackground: Leprosy is a chronic infectious disease caused by Mycobacterium leprae that can manifest a wide variety of immunological and clinical outcomes ranging from potent humoral responses among borderline lepromatous (BL) and lepromatous (LL) patients to strong cellular responses among tuberculoid (TT) and borderline tuberculoid (BT) patients. Until recently, relatively little has been known about the immune responses to individual proteins of M. leprae recognized during leprosy. Methods: The immune reactivity to a panel of 33 M. leprae recombinant proteins was evaluated among leprosy patients and controls from a high endemic area for leprosy (Goiania/GO, Central Brazil). Serum IgG responses were measured by ELISA (45 participants/group) and T cell responses (20 participants/group) were evaluated by IFN-gamma production in 24 hours whole blood cultures with antigen (whole blood assay-WBA). Study groups were newly diagnosed, untreated TT/BT and BL/LL leprosy patients classified by Ridley Jopling criteria and household contacts of BL/LL patients (HHC). Control groups were HIV-1 negative pulmonary tuberculosis patients (TB) and healthy individuals from the same endemic area (EC). In silico predictions indicated the level of identity of M. leprae proteins with homologues in other mycobacteria and the presence of T cell and B cell epitopes. Results: Despite the prediction that all proteins would be reactive, 16 of 33 (48%) of the single proteins tested were immunogenic (recognized in WBA or ELISA) and seventeen were non-immunogenic (not recognized in either assay). Among the 16 immunogenic proteins, 9 were considered leprosy specific in WBA inducing cell-mediated IFN-gamma secretion from TT/BT patients and HHC. Three of these proteins were also leprosy specific in serology being recognized by serum IgG from LL/BL patients. Seven of the immunogenic proteins were not leprosy specific. Conclusions: New M. leprae antigens recognized by antibody responses of BL/LL patients and cellular responses of TT/BT leprosy patients were identified. An improved serological diagnostic test for leprosy could be developed by incorporating these IgG-reactive antigens to the current PGL-I based tests. Moreover our data indicate that the WBA is a robust, relatively simple and user friendly format for a T cell based diagnostic test. The field use of these test formats in leprosy endemic countries could contribute to early leprosy diagnosis before the development of deformities and disabilities.pt_BR
dc.identifier.citationSAMPAIO, Lucas H.; STEFANI, Mariane M. A.; OLIVEIRA, Regiane M.; SOUSA, Ana L. M.; IRETON, Greg C.; REED, Steven G.; DUTHIE Malcolm S. Immunologically reactive M. leprae antigens with relevance to diagnosis and vaccine development. BMC Infectious Diseases, London, v. 11, n. 16, 2011.pt_BR
dc.identifier.doi10.1186/1471-2334-11-26
dc.identifier.issn1471-2334
dc.identifier.issne- 1471-2334
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/17041
dc.language.isoengpt_BR
dc.publisher.countryGra-bretanhapt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleImmunologically reactive M. leprae antigens with relevance to diagnosis and vaccine developmentpt_BR
dc.typeArtigopt_BR

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