Isolation and characterization of phenol degrading Bacillus species from a Southeast Brazilian mangrove sediment
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2018-12
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Resumo
Phenolic compounds are common pollutants in industrial effluents and pose a significant risk to the
environment. The microbial capacity to degrade these molecules makes the prospection of bacteria with
this ability very strategic for bioremediation processes in contaminated areas. This study was carried
out to isolate bacteria with phenol degrading capacity from Brazilian mangrove soil. Microorganisms
were evaluated for tolerance and the ability to use this substance as a single source of carbon. Two
bacterial isolates were obtained: MF-1 and MF-2. The microorganisms were identified, respectively, as
Bacillus cereus and Bacillus circulans by sequencing the 16S rRNA gene. The isolates exhibited
tolerance to phenol at concentrations up to 600 mg.L-1 when grown in Bushnell-Haas Agar (BHA) and
1,500 mg.L-1
in nutrient agar (NA). As for the use of phenol as a single carbon source, the
microorganisms were grown in concentrations of 100, 200 and 300 mg.L-1
of the compound. The isolate
MF-1 presented a percentage consumption of 22.43, 11.52 and 3.33%, respectively, for each tested
concentration. Although there was a decrease in phenol consumption in response to the increased
concentration of this substance, bacterial growth was not affected. The best results were obtained for
the isolate MF-2, in which consumption of phenol was, respectively, 21.54, 20.54 and 28.85% for these
concentrations. Likewise, the increase in phenol concentration caused no reduction in the growth of
this isolate.
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Bioremediation, Xenobiotic, Phenol degrading bacteria, Mangrove
Citação
ROTTA, Anna Paula Santos Almeida et al. Isolation and characterization of phenol degrading Bacillus species from a Southeast Brazilian mangrove sediment. African Journal of Microbiology Research, [s. l.], v. 12, n. 46, p. 1032-1038, 2018. DOI: 10.5897/AJMR2018.8860. Disponível em: https://academicjournals.org/journal/AJMR/article-abstract/6E49F9059669. Acesso em: 14 nov. 2024.