Morphometric analysis and virulence of isolates of toxoplasma gondii recovered from pregnant and newborn mice in Goiânia-GO

Resumo

Introduction: Studies in Toxoplasma gondii performed in newborns are interesting and relevant because they induce serious injuries in newborns when they are not properly treated. Objective: To perform morphometric analyses of Toxoplasma gondii isolated (Tachizoite forms), recovered from peritoneal exudation in mice infected with biological material recovered from pregnant women and newborns in Goiânia-GO. Methodology: Three isolates of Toxoplasma gondii in newborns and the RH lineage were evaluated as to the pathogenicity and virulence in Balb-c albino mice. The suspension of Taquizoito used in experimental tests was obtained by puncture or washing of the peritoneal cavity in mice with ascites. Each sample was inoculated into groups of four mice with inoculators of 10, 10, 10, 10, 10, 10 taquizoitos living, via intra peritoneum. Results and discussion: It was discovered that the isolates 15, 20 and RH lineage were lethal in mice. The isolate-3 behaved lethally in all mice up to 10 taquizoítos. The death of all animals that presented acute infection occurred 5-10 days after inoculation. Insulated-15, insulated-20 and RH strains showed greater diameter and smaller areas compared to the insulated-3. It turned out that the most virulent isolate also demonstrated greater area and diameter, and these findings reinforce the hypothesis that, in addition to behavioral differences and genetic variations, there are also morphological variations when different strains of T. gondii are evaluated. Final considerations: This new morphometric parameter corroborates the presence of diversity between isolated strains and may constitute new information used to characterize and analyze these strains.

Descrição

Palavras-chave

Toxoplasma gondii, Isolate, Newborn, Toxoplasma gondii, Isolar, Recém-nascido

Citação

SILVA, Marcos Gontijo da et al. Morphometric analysis and virulence of isolates of toxoplasma gondii recovered from pregnant and newborn mice in Goiânia-GO. Revista Amazônia Science & Health, Gurupi, v. 6, p. 48-53, 2018. DOI: 10.18606/2318-1419/amazonia.sci.health.v6n1p48-53. Disponível em: https://ojs.unirg.edu.br/index.php/2/article/view/2023. Acesso em: 30 abr. 2025.