Leprosy surveillance study in a highly endemic Brazilian area using leprosy specific serologic tests and IFNγ whole blood assay

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dc.creatorGonçalves, Aline do Carmo
dc.creatorPinto, Emerith Mayra Hungria
dc.creatorFreitas, Aline de Araújo
dc.creatorBührer, Samira
dc.creatorSantos, Cássia Maria Gomes dos
dc.creatorMelo, Ana Cecília Coelho
dc.creatorNascimento, Laura Branquinho do
dc.creatorStefani, Mariane Martins de Araújo
dc.date.accessioned2025-02-17T12:20:33Z
dc.date.available2025-02-17T12:20:33Z
dc.date.issued2020
dc.description.abstractThis surveillance study evaluated leprosy-serologic tests and the IFNγ whole-blood-assay/WBA as adjunct diagnostic tools. Previously diagnosed leprosy index cases, intradomiciliary, peridomiciliary contacts from a Brazilian endemic area were enrolled during domiciliary visits. Physical evaluation was performed by trained nurses and leprosy diagnosis confirmed by expert dermatologist. ELISA detected IgM anti-PGL-I, IgG anti-LID-1, and IgM/IgG anti-ND-O-LID antibodies. Heparinized WBA plasma stimulated with LID-1, 46f + LID-1, ML0276 + LID-1 (24 h, 37 °C, 5% CO2) was tested for human IFNγ (QuantiFERON®-TB Gold/QFT-G; Qiagen). The survey included 1731 participants: 44 leprosy index cases, 64 intradomiciliary, 1623 peridomiciliary contacts. Women represented 57.7%, median age was 32 years, 72.2% had BCG scar. Leprosy prevalence was higher in intradomiciliary (8.57%) versus peridomiciliary contacts (0.67%), p < 0.001. Among 23 suspects, five leprosy cases were confirmed: 4 multibacillary/MB and 1 paucibacillary/PB. Leprosy incidence was 0.30%: 1.56% in intradomiciliary versus 0.25% in peridomiciliary (p = 0.028). Seropositivity rates were 1.9% to PGL-I, 4.9% to LID-1, and 1.0% to ND-O-LID. LID-1 positivity was higher in all groups; incident cases were LID-1 seropositive. ND-O-LID positivity was higher in intra- versus peridomiciliary contacts (p = 0.022). IFNγ WBA (40 index cases, 19 suspects, 35 intradomiciliary, 74 peridomiciliary contacts) showed higher LID-1/WBA positivity in peridomiciliary contacts (p > 0.05); significant differences among groups were seen with 46f + LID-1 but 0276 + LID-1 induced higher IFNγ levels. Incident cases were LID-1 seropositive, while IFNγ-WBA had marginal diagnostic application. As seropositivity indicates exposed individuals at higher risk of disease development, the utility of serologic screening for surveillance and prophylactic measures remains to be demonstrated.
dc.identifier.citationGONCALVES, Aline do Carmo et al. Leprosy surveillance study in a highly endemic Brazilian area using leprosy specific serologic tests and IFNγ whole blood assay. European Journal of Clinical Microbiology & Infectious Diseases, Wiesbaden, v. 39, n. 12, p. 2345-2360, 2020. DOI: 10.1007/s10096-020-03979-5. Disponível em: https://link.springer.com/article/10.1007/s10096-020-03979-5. Acesso em: 11 fev. 2025.
dc.identifier.doi10.1007/s10096-020-03979-5
dc.identifier.issn0934-9723
dc.identifier.issne-1435-4373
dc.identifier.urihttps://link.springer.com/article/10.1007/s10096-020-03979-5
dc.language.isoeng
dc.publisher.countryAlemanha
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RMG)
dc.rightsAcesso Restrito
dc.titleLeprosy surveillance study in a highly endemic Brazilian area using leprosy specific serologic tests and IFNγ whole blood assay
dc.typeArtigo

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