Analysis of the secretomes of paracoccidioides mycelia and yeast cells

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dc.creatorWeber, Simone Schneider
dc.creatorParente, Ana Flávia Alves
dc.creatorBorges, Clayton Luiz
dc.creatorParente, Juliana Alves
dc.creatorBailão, Alexandre Melo
dc.creatorSoares, Célia Maria de Almeida
dc.date.accessioned2018-05-16T12:06:48Z
dc.date.available2018-05-16T12:06:48Z
dc.date.issued2012
dc.description.abstractParacoccidioides, a complex of several phylogenetic species, is the causative agent of paracoccidioidomycosis. The ability of pathogenic fungi to develop a multifaceted response to the wide variety of stressors found in the host environment is important for virulence and pathogenesis. Extracellular proteins represent key mediators of the host-parasite interaction. To analyze the expression profile of the proteins secreted by Paracoccidioides, Pb01 mycelia and yeast cells, we used a proteomics approach combining two-dimensional electrophoresis with matrix-assisted laser desorption ionization quadrupole time-of-flight mass spectrometry (MALDI-Q-TOF MS/MS). From three biological replicates, 356 and 388 spots were detected, in mycelium and yeast cell secretomes, respectively. In this study, 160 non-redundant proteins/isoforms were indentified, including 30 and 24 proteins preferentially secreted in mycelia and yeast cells, respectively. In silico analyses revealed that 65% of the identified proteins/isoforms were secreted primarily via non-conventional pathways. We also investigated the influence of protein export inhibition in the phagocytosis of Paracoccidioides by macrophages. The addition of Brefeldin A to the culture medium significantly decreased the production of secreted proteins by both Paracoccidioides and internalized yeast cells by macrophages. In contrast, the addition of concentrated culture supernatant to the co-cultivation significantly increased the number of internalized yeast cells by macrophages. Importantly, the proteins detected in the fungal secretome were also identified within macrophages. These results indicate that Paracoccidioides extracellular proteins are important for the fungal interaction with the host.pt_BR
dc.identifier.citationWEBER, Simone Schneider et al. Analysis of the secretomes of paracoccidioides mycelia and yeast cells. Plos One, San Francisco, v. 7, n. 12, e52470, 2012.pt_BR
dc.identifier.doi10.1371/journal.pone.0052470
dc.identifier.isbn1932-6203
dc.identifier.issne- 1932-6203
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/14979
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleAnalysis of the secretomes of paracoccidioides mycelia and yeast cellspt_BR
dc.typeArtigopt_BR

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