Resistance profile of P. aeruginosaisolates to β-lactamsantimicrobials

Abstract

β-lactamasesare enzymes that hydrolyze the β-lactam ring, inactivating the action of β-lactam antibiotics. The objective of this work was to diagnosephenotypically and molecularly 14β-lactamase resistance genes expressed in Pseudomonas aeruginosaand to correlate the results found.A total of 99 samples of Pseudomonas aeruginosawere selected and the antibiogram was performed. Real-time PCR is being performed using the Sybr Green system to amplify the genes corresponding to the resistances found in phenotyping.Of the 99 samples, 14 were identified as phenotypically resistant to the antimicrobial Aztreonam. The blaSME, blaOXA, blaGIM genes were simultaneously found in three (21.4%) samples. According to the statistical test, when evaluating the amplification results obtained for piperacillin + tazobactam, the molecular method was more sensitive for the detection of the gene coding for multidrug resistance, presenting values of (p <0.05).This information suggests gene researches aremore sensitive and specific when compared with to the antibiogramtest.