Fisetin as a promising antifungal agent against Cryptocococcus neoformans species complex
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Data
2016
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Aims
The aim of this study was to investigate the mechanisms of action of fisetin, a flavonol with antifungal activity previously evaluated against the Cryptococcus neoformans species complex.
Methods and Results
Ergosterol content and flow cytometry analysis were determined for the C. neoformans species complex in the presence of fisetin and ultrastructural analysis of morphology was performed on Cryptococcus gattii and C. neoformans. Decrease in the total cellular ergosterol content after exposure to fisetin ranged from 25·4% after exposure to 128 μg ml−1 to 21·6% after exposure to 64 μg ml−1of fisetin compared with the control (without fisetin). The fisetin effects obtained with flow cytometry showed metabolic impairment, and alterations in its normal morphology caused by fisetin in C. neoformans cells were verified using scanning electron microscopy.
Conclusions
Fisetin is a compound that acts in the biosynthesis of ergosterol. Flow cytometry showed that fisetin reduced viability of the metabolically active cells of C. gattii, while morphological changes explain the action of fisetin in inhibiting growth of these fungi.
Significance and Impact of the Study
This study supports the idea that fisetin may represent a good starting point for the development of future therapeutic substances for cryptococcosis.
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Antifungal activity, Cryptococcus neoformans, Fisetin, Mechanisms of action, Scanning electron microscopy
Citação
REIS, M. P. C. et al. Fisetin as a promising antifungal agent against cryptococcus neoformans species complex. Journal of Applied Microbiology, Oxford, v. 121, n. 2, p. 373-379, 2016. DOI: 10.1111/jam.13155. Disponível em: https://academic.oup.com/jambio/article/121/2/373/6717309?login=true. Acesso em: 5 jun. 2025.