Leishmania (Viannia) braziliensis amastigotes from patients with mucosal leishmaniasis have increased ability to disseminate and are controlled by nitric oxide at the early stage of murine infection
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2016
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Mucosal leishmaniasis (ML) caused by Leishmania (Vianna) braziliensis usually appears after the healing of the primary lesion
when amastigotes disseminate from the infection site to the mucosal area. Here, we investigated murine infection with
amastigotes obtained from patients with ML or localized cutaneous leishmaniasis (LCL). Amastigotes were used to infect
wild type, IFN-γ KO and inducible nitric oxide synthase (iNOS) KO mice. Amastigotes from patients with LCL induced
lesions that appeared earlier in IFN-γ KO than parasites from ML. The lesion after infection with ML appeared early in iNOS
KO than in IFN-γ KO mice and in iNOS KO mice parasites from ML and LCL cause similar lesions at the initial phase of
infection, while parasites from ML induced greater lesions than the ones from LCL at the late phase. A greater number of
parasites were observed in spleen of IFN-γ KO and iNOS KO mice infected with amastigotes from patients with ML than
those with LCL. Parasites from ML infect a lower percentage of macrophages and are killed independent on IFN-γ and
dependent on NO. The data suggest that amastigotes responsible for mucosal lesion in humans develop slowly on the
initial phase of infection due to high susceptibility to NO and they have an increased ability to disseminate.
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Leishmania (V.) braziliensis, Amastigote, Nitric oxide, Mucosal leishmaniasis, Interferon-gamma
Citação
GOMES, Clayson M. et al. Leishmania (Viannia) braziliensis amastigotes from patients with mucosal leishmaniasis have increased ability to disseminate and are controlled by nitric oxide at the early stage of murine infection. Pathogens and Disease, Oxford, v. 74, n. 4, eftw023-9, 2016. DOI: 10.1093/femspd/ftw023. Disponível em: https://academic.oup.com/femspd/article/74/4/ftw023/2197834?login=true. Acesso em: 20 mar. 2025.