Disruption of calcium homeostasis in cardiomyocytes underlies cardiac structural and functional changes in severe sepsis

dc.creatorCeles, Mara Rúbia Nunes
dc.creatorMalvestio, Lygia Maria Mouri
dc.creatorSuadicani, Sylvia O.
dc.creatorPrado, Cibele Maria
dc.creatorFigueiredo, Maria José
dc.creatorPulici, Érica Carolina Campos
dc.creatorFreitas, Ana Caroline Silva de
dc.creatorSpray, David C.
dc.creatorTanowitz, Herbert B.
dc.creatorSantos, José Sebastião dos
dc.creatorRossi, Marcos Antonio
dc.date.accessioned2019-01-24T11:20:55Z
dc.date.available2019-01-24T11:20:55Z
dc.date.issued2013-07-23
dc.description.abstractSepsis, a major cause of morbidity/mortality in intensive care units worldwide, is commonly associated with cardiac dysfunction, which worsens the prognosis dramatically for patients. Although in recent years the concept of septic cardiomyopathy has evolved, the importance of myocardial structural alterations in sepsis has not been fully explored. This study offers novel and mechanistic data to clarify subcellular events that occur in the pathogenesis of septic cardiomyopathy and myocardial dysfunction in severe sepsis. Cultured neonatal mice cardiomyocytes subjected to serum obtained from mice with severe sepsis presented striking increment of [Ca2+]i and calpain-1 levels associated with decreased expression of dystrophin and disruption and derangement of F-actin filaments and cytoplasmic bleb formation. Severe sepsis induced in mice led to an increased expression of calpain-1 in cardiomyocytes. Moreover, decreased myocardial amounts of dystrophin, sarcomeric actin, and myosin heavy chain were observed in septic hearts associated with depressed cardiac contractile dysfunction and a very low survival rate. Actin and myosin from the sarcomere are first disassembled by calpain and then ubiquitinated and degraded by proteasome or sequestered inside specialized vacuoles called autophagosomes, delivered to the lysosome for degradation forming autophagolysosomes. Verapamil and dantrolene prevented the increase of calpain-1 levels and preserved dystrophin, actin, and myosin loss/reduction as well cardiac contractile dysfunction associated with strikingly improved survival rate. These abnormal parameters emerge as therapeutic targets, which modulation may provide beneficial effects on future vascular outcomes and mortality in sepsis. Further studies are needed to shed light on this mechanism, mainly regarding specific calpain inhibitors.pt_BR
dc.identifier.citationCELES, Mara R. N.; MALVESTIO, Lygia M.; SUADICANI, Sylvia O.; PRADO, Cibele M.; FIGUEIREDO, Maria J.; CAMPOS, Erica C.; FREITAS, Ana C. S.; SPRAY, David C.; TANOWITZ, Herbert B.; SILVA, João S. da; ROSSI, Marcos A. Disruption of calcium homeostasis in cardiomyocytes underlies cardiac structural and functional changes in severe sepsis. Plos One, San Francisco, v. 8, n. 7, e68809, 2013.pt_BR
dc.identifier.doi10.1371/journal.pone.0068809
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/16828
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.titleDisruption of calcium homeostasis in cardiomyocytes underlies cardiac structural and functional changes in severe sepsispt_BR
dc.typeArtigopt_BR

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