Intranasal vaccine using P10 peptide complexed within chitosan polymeric nanoparticles as experimental therapy for Paracoccidioidomycosis in murine model
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Data
2020
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Resumo
Paracoccidioidomycosis (PCM) is a granulomatous fungal disease caused by the dimorphic
fungal species of Paracoccidioides, which mainly affects the lungs. Modern strategies for the treatment
and/or prevention of PCM are based on a Th1-type immune response, which is important for
controlling the disease. One of the most studied candidates for a vaccine is the P10 peptide, derived
from the 43 kDa glycoprotein of Paracoccidioides brasiliensis. In order to improve its immune modulatory
effect, the P10 peptide was associated with a chitosan-conjugated nanoparticle. The nanoparticles
presented 220 nm medium size, poly dispersion index (PDI) below 0.5, zeta potential of +20 mV
and encapsulation efficiency around 90%. The nanoparticles’ non-toxicity was verified by hemolytic
test and cell viability using murine macrophages. The nanoparticles were stable and presented
physicochemical characteristics desirable for biological applications, reducing the fungal load and the
usual standard concentration of the peptide from 4 to 20 times.
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Paracoccidioidomycosis, P10 peptide, Polymeric nanoparticles, Nanovaccine, Antifungal therapy
Citação
SANTOS JUNIOR, Samuel Rodrigues dos et al. Intranasal vaccine using P10 peptide complexed within chitosan polymeric nanoparticles as experimental therapy for Paracoccidioidomycosis in murine model. Journal of Fungi, Basel, v. 6, n. 3, e160, 2020. DOI: 10.3390/jof6030160. Disponível em: https://www.mdpi.com/2309-608X/6/3/160. Acesso em: 7 maio 2025.