Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil
Nenhuma Miniatura disponível
Data
2020
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including
Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While
MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the his torical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been
reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome
against the published ones. A female patient who had never visited Africa presented with clinical symptoms
typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to
MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping
based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The
isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF
genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome
(MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an
African index case.
Descrição
Palavras-chave
Tuberculosis, Mycobacterium tuberculosis var. africanum, Brazil, Molecular epidemiology, Genome, Case repor
Citação
RABAHI, Marcelo Fouad et al. Characterization of mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil. Infection, Genetics and Evolution, Amsterdam, v. 85, e104550, 2020. DOI: 10.1016/j.meegid.2020.104550. Disponível em: https://www.sciencedirect.com/science/article/pii/S1567134820303816. Acesso em: 20 ago. 2024.