Antifungal potential of punicalagin against Cryptococcus neoformans species complex
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2018
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This study evaluated the antifungal activity and cytotoxicity profile of the ellagitannin
punicalagin, a compound extracted from the L. pacari A.St.-Hil (Lythraceae) leaf, against
Cryptococcus neoformans species complex. Minimum inhibitory concentrations (MIC) were
checked using the broth microdilution method. Minimum fungicidal concentrations (MFC)
and time of death were used to confirm the antifungal activity of the compound. The in vitro
cytotoxicity of punicalagin was tested in BALB/c3T3 fibroblasts and A549 human lung cancer
cell line, while the hemolytic potential was tested on sheep erythrocytes. The morphological
changes induced in yeast strains by the presence of punicalagin were also analyzed. Tested
on eight isolates of the C. neoformans complex punicalagin showed MIC of 0.5 to 4.0 μg/mL
and MFC> 256 μg/mL. Punicalagin also demonstrated a good growth inhibitory activity in
time-kill curves, but it was not able to achieve a statistically significant reduction of fungal
growth suggesting a fungistatic effect of the compound. In vitro cytotoxicity studies using the
two cell lines showed that punicalagin has low activity on these cells and no activity on sheep
erythrocytes. Morphological changes were seen in the yeasts strains studied when treated
with punicalagin. Therefore, punicalagin is a potential antifungal for important pathogenic
yeasts and presents a low cytotoxicity profile associated with no hemolytic effects.
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Punicalagin, Lafoensia pacari A.St.-Hil, Cytotoxicity, Antifungal activity, Cryptococcus neoformans species complex
Citação
SILVA, Thaísa Cristina et al. Antifungal potential of punicalagin against Cryptococcus neoformans species complex. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 60, e60, 2018. DOI: 10.1590/S1678-9946201860060. Disponível em: https://www.scielo.br/j/rimtsp/a/6RMVpcVnGHcJg4T6ks8LNzJ/abstract/?format=html&lang=en. Acesso em: 5 jun. 2025.