New insight on the acute CCl4-induced hepatotoxicity model in rats

dc.creatorMelo, Dorcas Fernandes dos Anjos
dc.creatorSilva, Marina Alves Coelho
dc.creatorOliveira, Naiara Raica Lopes de
dc.creatorOliveira Neto, Jerônimo Raimundo de
dc.creatorLino Junior, Ruy de Souza 
dc.creatorCruz, Alessandro Carvalho
dc.creatorCunha, Luiz Carlos da
dc.date.accessioned2025-09-26T13:27:56Z
dc.date.available2025-09-26T13:27:56Z
dc.date.issued2025
dc.description.abstractThe CCl4-induced hepatotoxicity model is a traditional preclinical assay applied to evaluate potential hepatoprotective compounds. However, several studies have used it with inappropriate dose and exposure time, generating both weak response or irreversible liver injury, as well as lack of representative liver and plasma biomarkers. Therefore, this study aims to determine the best dose and exposure time of CCl4 in Wistar rats, permitting a proper evaluation of potential hepatoprotective effect. Thus, CCl4-intraperitoneal doses of 0.5, 1.0, and 2.0 mL/kg were first evaluated 24 h post-exposure, and then with the best dose achieved, it was also assessed at 6 and 12 h post-exposure. The determination of the main hepatotoxicity biomarkers, including malondialdehyde (MDA), aspartate transaminase (AST), and alanine transaminase (ALT), and histopathological analyses were performed. The results suggest that 6h CCl4 post-exposure is too short to induce ideal liver injury, and at 24 h, a suggestive rat free-radical scavenger mechanism seems to revert CCl4-initiated damage. According to these data, the ideal acute CCl4-induced hepatotoxicity model was established at a dose of 2.0 mL/kg and 12 h post-exposure in Wistar rats, which demonstrated a significant increase of liver MDA levels without irreversible injury, permitting a proper and reliable evaluation of potential hepatoprotective compounds.
dc.identifier.citationMELO, Dorcas Fernandes dos Anjos et al. New insight on the acute CCl4-induced hepatotoxicity model in rats. Naunyn-Schmiedebergs Archives of Pharmacology, Berlin, v. 398, n. 6, 2025, p. 7643-7648, 2025. DOI: 10.1007/s00210-025-03824-6. Disponível em: https://link.springer.com/article/10.1007/s00210-025-03824-6. Acesso em: 25 set. 2025.
dc.identifier.doi10.1007/s00210-025-03824-6
dc.identifier.issn0028-1298
dc.identifier.issne- 1432-1912
dc.identifier.urihttps://link.springer.com/article/10.1007/s00210-025-03824-6
dc.language.isoeng
dc.publisher.countryAlemanha
dc.publisher.departmentInstituto de Patologia Tropical e Saúde Pública - IPTSP (RMG)
dc.rightsAcesso Restrito
dc.subjectAcute preclinical model
dc.subjectCCl4-induced hepatotoxicity
dc.subjectHepatoprotective evaluation
dc.subjectMalondialdehyde biomarker
dc.titleNew insight on the acute CCl4-induced hepatotoxicity model in rats
dc.typeArtigo

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