Mestrado em Ciência Animal (EVZ)
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Item Caracterização de extratos de própolis e efeito sobre Campylobacter termotolerantes de origem avícola(Universidade Federal de Goiás, 2020-02-29) Calazans, Luíza Toffano Seidel; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Bruna Aparecida Souza; http://lattes.cnpq.br/4021221951532780; Rezende, Cíntia Silva Minafra e; http://lattes.cnpq.br/5841210447886226; Rezende, Cíntia Silva Minafra e; Teixeira, Weslen Fabrício Pires; Tenório, Clarice Gebara Muraro Serrate CordeiroThermotolerants Campylobacter are related to a group of pathogens that are carried by food and are currently considered as the main ones of global occurrence and with high frequency of resistance to antimicrobials. From the above, the present study aimed to identify biocomposites present in four extracts of green and red propolis and to evaluate alternatives regarding the antimicrobial potential. Extracts from Brazil, obtained by conventional ethanol extraction and extraction assisted by ultrasound, were evaluated. Four cultures of collections and twenty-five isolates from three species (Campylobacter jejuni, Campylobacter coli and Campylobacter lari) comprised the study. The technique used was microdilution in broth in a 96-well plate, which allowed the analysis of eleven concentrations of the propolis extracts (0.01 to 100 mg / mL-1). Variability was observed for antimicrobial activity. Greater microbial viability and motility were identified by optical microscopy at the highest concentrations of propolis tested. In addition, regarding the effect on bacterial viability of red propolis in relation to green propolis, the red one showed more efficient. There was no difference in extraction methods. It is concluded that the propolis extracts had an unsatisfactory antimicrobial effect, as well as a cellular protective effect for high concentrations in this study, regardless of the extraction method, which may have a desirable connotation for laboratory diagnosis.Item Fatores de risco para infecção pelos vírus da diarreia viral e rinotraqueíte infecciosa bovina no Sítio Histórico e Patrimônio Cultural Kalunga(Universidade Federal de Goiás, 2016-03-04) Dias, Juliana Moraes; Arnhold, Emmanuel; http://lattes.cnpq.br/7156945506134934; Juliano, Raquel Soares; http://lattes.cnpq.br/4884381347303453; Fioravanti, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; Fioravanti, Maria Clorinda Soares; Pimentel, Concepta Margaret McManus; Moura, Maria Ivete deThe research consisted of evaluating the frequency of seropositive animals for viral diarrhea and infectious bovine rhinotracheitis and determining correlations with environmental, social and health characteristics of the region. The study was carried out in herds of the Kalunga’s Historical and Cultural Heritage Site, located in the north of the state of Goiás, it is an area of Cerrado with native pastures and few formed areas. Blood samples were collected from 2,612 cattle of different breeds and age groups, male and female, corresponding to 79 predominantly beef cattle. During sample collection, an epidemiological questionnaire was applied to investigate factors that could be associated with infections. The samples were submitted by the ELISA method, to detect antibodies against BVD and IBR. The average frequency of seropositive herds was 88.6% for BVD and 97% for IBR, the individual positive serology corresponded to 17.8% for BVD and 45.5% for IBR. There was a significant difference (p <0.05) between the regions and age group for both diseases. The environmental variables associated with the frequency of BVD were: level of precipitation and maximum temperature of the hottest month of the year, and for IBR: INDV and temperature seasonality. Contact with other species of animals, reproductive problems, presence of wetland, use of cattle as the main source of income, educational level of the producer and marital status were correlated risk factors for the occurrence of BVD and IBR.Item Função sistólica do ventrículo esquerdo em cães com doença mixomatosa valvar mitral(Universidade Federal de Goiás, 2019-12-27) Sousa, Paulo Roberto de; Damasceno, Adilson Donizeti; http://lattes.cnpq.br/3900110295277130; Torres, Bruno Benetti Junta; http://lattes.cnpq.br/7060242425070126; Carvalho, Rosângela de Oliveira Alves; http://lattes.cnpq.br/5593911382353878; Carvalho, Rosângela de Oliveira Alves; Sousa, Marlos Gonçalves; Damasceno, Adilson DonizetiMyxomatous mitral valve disease (MMVD), or mitral valve endocardiosis, is the most prevalent acquired heart disease in dogs, being the main cause of heart failure in small dogs. The echocardiographic examination, which allows cardiac morphological and functional evaluation, is considered the “gold standard” test for diagnosing and staging MMVD in dogs. Information obtained by echocardiography on ventricular function also determines the prognosis in dogs with MMVD, since systolic dysfunction is a risk factor in these patients and contributes to the development of heart failure. However, the assessment of ventricular systolic function using conventional echocardiographic indexes is limited, since it is directly influenced by preload and afterload factors. The evaluation of cardiac mechanics by strain (ST) and strain rate (STR), using two-dimensional speckle tracking (2D-STE), allows, in humans, an earlier diagnosis of ventricular dysfunction. In addition, the mitral ring tissue displacement (TMAD) is an index of longitudinal systolic function that has a good correlation with conventional echocardiographic indices and low intra and interobserver variability. Thus, the objective of this study was to verify whether the myocardial deformity indexes (ST and STR) and the TMAD are more accurate and precocious than the conventional echocardiographic indexes in the assessment of left ventricular systolic function in dogs with MMVD, measuring the variables in the day of diagnosis (T0) and after 30 days (T30). For this purpose, 29 dogs were evaluated, categorized according to the ACVIM consensus, eight of which were in stage B1, 12 in stage B2 and nine in stage C. Considering the conventional variables used in this study, the presence of systolic dysfunction was not observed in dogs with MMVD, however changes after the start of pharmacological treatment were observed in the variables FET% and MAM. From the 2D-STE evaluation, similar to the conventional evaluation, systolic dysfunction cannot be determined in dogs with MMVD. However, the longitudinal ST variable indexed by weight showed a significant increase between the assessment times in group C, being still significantly higher in C than in B2, at administration, and higher in C than in the other groups after treatment. For the variable longitudinal STR it decreased in T30 in B1 and increased in C. When comparing the groups, in T30, group C showed higher values than in B1 and B2. Still, the variables longitudinal ST and TMAD showed a significant negative correlation when indexed to weight. In conclusion, the assessment of myocardial deformity using 2D-STE assessment did not demonstrate superiority compared to conventional indices for the diagnosis of systolic dysfunction in dogs with MMVD in its different stages.Item Ultrassonografia da articulação femorotibiopatelar de ovinos com sinovite experimental(Universidade Federal de Goiás, 2019-01-17) Souza, Michel Felipe Soares; Borges, Naida Cristina; http://lattes.cnpq.br/9181279951885005; Silva, Marco Augusto Machado; http://lattes.cnpq.br/2134206254307043; Silva, Marco Augusto Machado; Borges, Naida Cristina; Silva, Marcelo Seixo de Brito e; Cardoso, Júlio Roquete; Souza, Luiz Augusto deThe ovine stifle joint is complex and multiple bone and soft tissue elements comprise its anatomy. It is used as an experimental model for human stifle joint disease studies. Articular ultrasonography is an important diagnostic technique for articular diseases. However, there is a gap in the literature regarding ultrasound assessment of the ovine stifle joint as an experimental model. Thus, the purpose of this study was to describe sonographic changes of the ovine stifle joint undergone experimentally induced synovitis. Twelve ovine received intra-articular infusion of E. coli lipopolysaccharide in the right stifle joint to induce synovitis. Injected stifles were assessed by ultrasound at baseline and 12, 24, 48, 72 and 120 hours after intra-articular injection of lipopolysaccharide. Nine ultrasound positions were used to assess the stifle joint: suprapatelar area; lateral area of the femorotibial compartment; infrapatelar space; medial area of the femorotibial compartment; lateral femoropatelar compartment; medial femoropatelar compartment; craniolateral area; sheath of the origin of the extensor muscle tendons; and medial supracondilar recess. Stifle joint was evaluated for volume changes in the synovial fluid, thickness of the synovial membrane and cellularity. Intra-articular lipopolysaccharide injection yielded echographic changes in seven out of nine scanning positions at the ovine stifle joint. Changes were observed when the medial femorotibial compartment was evaluated in 11 animals (91.7%), in the craniolateral region, in 10 animals (83,34%), in the tendinous recess, in 11 animals (91.7%), and in the infrapatellar space, lateral and medial aspects of the femoropatellar compartments and medial supracondilar recess in all animals (100%). It was not observed alterations within the suprapatellar region and the lateral femorotibial compartment. Stifle joint ultrasound assessment was efficient for detection of intra-articular changes following infiltration with lipopolysaccharide in the ovine experimental model. The most observed signs were increase of the synovial fluid volume and thickening of the synovial membrane, 12, 24 and 48 hours after infiltration.Item Comparação dos métodos de controles estratégico e seletivo na carga parasitária de Rhipicephalus microplus em rebanhos bovinos leiteiros(Universidade Federal de Goiás, 2020-03-02) Vieira, Luciano da Silva; Cotta, Viviane Zeringóta Rodrigues; http://lattes.cnpq.br/4269635925947346; Monteiro, Caio Márcio de Oliveira; http://lattes.cnpq.br/2496419306394657; Borges, Lígia Miranda Ferreira; http://lattes.cnpq.br/2591106734434348; Borges, Lígia Miranda Ferreira; Cotta, Viviane Zeringóta Rodrigues; Monteiro, Caio Márcio de Oliveira; Louly, Carla Cristina Braz; Lopes, Wélber Daniel ZanettiRhipicephalus microplus is one of the main ectoparasites of economic importance for cattle in tropical and subtropical regions, causing drop in milk production and transmitting pathogenic agents such as Anaplasma and Babesia. Therefore, constant studies on treatment schemes for parasite control are necessary to minimize the damage and losses that follow infestations by this arthropod. In this way, the present study aimed to compare two methods for R. microplus control, the strategic method and the selective method, in dairy cattle herds, aiming to provide support to the technical guidelines on the most efficient control method of the parasitic load. In the strategic method, seven treatments with spray acaricides were carried out every 21 days, starting in November. In the selective method, treatments with acaricides were performed only on animals that had an infestation equal to or greater than 40 engorged females. Only 5% of animals submitted to the selective method received treatment in November. The average of tick counts was lower in the strategic method compared to the selective method, in December. The maximum peak of infestation occurred in January, in both control methods, with no significant difference, persisting until February. No animal of the strategic method had an infestation greater than 40 engorged females in March. However, the parasitic load in both control methods was similar in the second evaluation in March. The averages of counts in the strategic method were lower in the months of April (10.9) and May (16.9) compared to the averages observed in the selective method (56.3 and 48.4). Yet, it was necessary to carry out tactical treatments in the strategic method, after the treatment scheme, in up to 16.7% of the animals in April and up to 50.0% in May, while in the selective method, the treatments varied from 42.8 to 88.8% in April and in May it varied from 28.6 to 75.0%. The total count of R. microplus was 5,036 engorged females in the strategic method, while in the selective method there were 13,829 engorged females in the study period. Therefore, it is concluded that the treatment scheme for control of R. microplus significantly interfered in the parasitic load of dairy cows, favouring the maintenance of acceptable levels of parasitism when the strategic method of tick control was implemented compared to the adoption of the selective method.