Repositório de teses e dissertações da Universidade Federal de Goiás

Comunidades no DSpace

Selecione uma comunidade para navegar por suas coleções

Agora exibindo 1 - 2 de 2

Campus Goiás (CG) 0
Região Metropolitana de Goiânia (RMG) 13051
Campus Samambaia, Campus Colemar Natal e Silva, Campus Aparecida de Goiânia.

Submissões Recentes

Fabricação de sensores eletroquímicos e dispositivos de fluxo lateral para aplicações bioanalíticas
(Universidade Federal de Goiás, 2026-02-17) Pereira, Mauricio Matheus Lucio; Coltro, Wendell Karlos Tomazelli; http://lattes.cnpq.br/8302650734477213; Paixão, Thiago Regis Longo Cesar da; Sgobbi, Livia Florio; Coltro, Wendell Karlos Tomazelli
This work presents the development and evaluation of low-cost analytical platforms with potential for portable applications and point-of-care (POC) analysis. Initially, an electrochemical sensor based on conductive tracks directly produced on glass substrates using a laser engraving technique was developed. The fabrication parameters were systematically optimized, and the optimal conditions were established as a power of 2.7 J s⁻¹, a speed of 3.6 mm s⁻¹, and a laser distance of 6 mm, resulting in tracks with minimum electrical resistivity in the range of 27–41 Ω cm. Structural characterization by Raman spectroscopy confirmed the formation of carbonaceous material with graphitic character, evidenced by well-defined D and G bands and a reduction in the ID/IG ratio after chemical treatment with acetic acid, indicating greater structural organization and an increase in sp² domains. FE-SEM analyses revealed a porous and rough surface with a higher density of cavities after acid treatment. The electrochemical performance was evaluated by cyclic voltammetry using the redox probe [Fe(CN)₆]³⁻/⁴⁻, showing reversible behavior, good repeatability, and a diffusion-controlled response. The analytical application of the sensor was investigated for the detection and quantification of midazolam maleate (MID) using differential pulse voltammetry and square wave voltammetry, with the former showing the best performance. The sensor exhibited a linear response in the range of 25 to 150 mg L⁻¹, with limits of detection and quantification of 2.9 mg L⁻¹ and 8.9 mg L⁻¹, respectively. Interference studies demonstrated a low influence of common compounds, and application in real samples of water and fortified alcoholic beverages showed recoveries between 88 and 112%, confirming the potential of the platform for rapid, portable, and lowcost forensic analyses. Additionally, a lateral flow-based analytical device was developed aiming to construct a simple and accessible system for POC diagnostic applications. The device was assembled through the sequential overlap of a glass fiber sample/conjugate pad, a nitrocellulose membrane, and an absorbent pad, allowing continuous and reproducible capillary flow. Different methodologies for depositing test and control lines were evaluated, including manual and semiautomated approaches, revealing reproducibility limitations associated with manual deposition methods. As a molecular recognition strategy, the biotin–streptavidin interaction was explored, involving the immobilization of streptavidin on the membrane and the capture of biotinylated amplicons, whose interaction was confirmed by fluorescence. Furthermore, a controlled deposition system using a linear displacement stage coupled with a syringe pump was developed, enabling greater control in reagent deposition. The results demonstrate the potential of the lateral flow device and the developed deposition system, as well as the need for additional optimization steps to improve line deposition and the analytical performance of the device. Overall, the results highlight the potential of different fabrication strategies for low-cost analytical devices, emphasizing both laser-engraved electrochemical sensors and lateral flow devices as promising alternatives for rapid, portable, and accessible analytical applications.
Valorização das culturas nas aulas de educação física: um caminho para a redução de conflitos e fortalecimento das identidades
(Universidade Federal de Goiás, 2026-03-31) Gobbi, Honorina Luiz Torres de Lima; Zandomínegue, Bethânia Alves Costa; https://lattes.cnpq.br/7739333559051242; Bandeira, Lilian Brandão; Barbosa, Raquel Firmino Magalhães; Zandomínegue, Bethânia Alves Costa
This dissertation focuses on the pedagogical practice of Physical Education in relation to cultures. The study is guided by the following questions: What are the students' perceptions regarding the role of Physical Education in improving the school environment and reducing conflicts? In what ways can Physical Education pedagogical practices that recognize and value student cultures influence conflict mitigation and promote a school climate more conducive to socialization? These questions underpin the objectives of: a) identifying students' perceptions regarding the role of Physical Education in improving the school environment; b) analyzing how valuing student cultures in Physical Education classes can contribute to a more inclusive and safe school environment; and c) developing and implementing a Physical Education action plan that encourages joint student participation, documenting examples of cultural pedagogical practices in Physical Education and their impact on the school environment. The research was conducted with 8th-grade Middle School students at a state public school in the city of Caldas Novas, Goiás. The study followed action research principles and used questionnaires, interviews, field note observations, and focus groups as data collection instruments. The research findings point out that pedagogical practices drawing on students' experiences and cultural backgrounds build up engagement, a sense of belonging and school ties. The experiences bear out that Physical Education can help work through conflicts and improve the school environment when it hinges on dialogue, listening and working together.
O ódio que você semeia: uma análise da construção argumentativa das emoções em discursos de ódio a partir do webtoon sul-coreano Dark Heaven
(Universidade Federal de Goiás, 2026-02-25) Oliveira, Iara; Morais, Rubens Damasceno; http://lattes.cnpq.br/6418455746725194; Lima, Helcira Maria Rodrigues de; Plantin, Christian
This dissertation aims to analyze, from the perspective of argumentation and emotion studies, how hate proliferates through argumentative interaction between characters in a South Korean fictional narrative: Dark Heaven. The work is a webtoon set in the imaginary European country of Van Tescosa, where the rise of a white supremacist group exposes an institutionalized culture of violence and exclusion against immigrants and queer people. In order to unveil the interactive and emotional layers of the hate speech uttered by the characters, we draw on the Dialogical Model of Argumentation (Plantin, 2008; 2025), centered on argumentative roles (Proponent, Opponent, Third party) and on the argumentative question under debate, as well as on Plantin’s theory of emotional arguments (1998; 2011), which enables the discursive reconstruction of affects based on linguistic and semiotic cues. Our theoretical framework on emotions relies mainly on the contributions of Plantin (1998; 2011), Aristotle (2005), Micheli (2010) and Lima (2023; 2024; 2025), while the discussion of hate speech engages with the notions of conspiracism/conspiracy thinking (Lima, 2023; Angenot, 2008) and the imaginary of tradition (Charaudeau, 2015), used in certain contexts to justify hate discourse. The understanding of the webtoon as a discursive, digital and multimodal genre is grounded in authors such as Bakhtin (2011), Pegoraro (2024) and Ramos (2009). Methodologically, the corpus consists of two specific excerpts, named “WAF” and “Matka”. The main analytical categories mobilized are argumentative roles, argumentative question, topoi, emotion frames and experiencer, articulated in an analytical framework we call the Topical System of Emotions in Interaction, an attempt to combine the core categories of both of Plantin’s theoretical-methodological models. Due to the strong multimodality of webtoons, we developed a color-coded legend to map resources of emotional reconstruction and intensification in the excerpts, incorporating semiotic elements such as framing, speech balloons, facial expressions, onomatopoeia and others into the analysis. The results indicate that hate operates as a discursive effect produced by argumentative strategies and by the mobilization of emotions such as fear and indignation. Furthermore, the findings show that hate is sustained by argumentative-emotional cycles. In the analyzed excerpts, discourses that construct foreigners and homosexuals as a collective threat activate fear and a sense of loss of tradition, thereby “justifying” preventive violence. The fear mobilized by the Proponent is transferred to and amplified within the audience, which may shift from spectator to potential agent of violence. In this way, the study demonstrates that hate speech not only expresses emotions but strategically produces and distributes them, and that argumentative interaction creates affective continuities that keep hate in circulation. Motivated by the possible relations between argumentation and emotions, this research seeks to foreground the pathemic dimension of discourse and argumentation, thus contributing to argumentation studies. By discussing these relations through a fictional text, the dissertation also aims to promote the webtoon genre, still little known and researched in Brazilian academia, and to challenge the apparent impossibility of addressing real social problems in linguistic studies through fiction
Racismo estrutural e mercado de trabalho : uma análise sociológica do desenvolvimento de carreira de mulheres negras no Banco Itaú na cidade de Goiânia
(Universidade Federal de Goiás, 2026-05-08) Lima, Leandro da Silva; Vettorassi, Andréa; http://lattes.cnpq.br/4314274220564712; Vettorassi, Andrea; Oliveira, Jaqueline Pereira De; Simoni, Rosinalda Corrêa da Silva
This study aims to analyze the career development of black women at Banco Itaú, in Goiânia, focusing on the implications of structural racism in the labor market. The research aims to understand how institutional practices and structural prejudices affect the professional advancement of these women, exploring factors such as socioeconomic profile, academic background, and professional history. The central question of the research is: how does structural racism impact the career development of black women in the banking sector? To this end, the research aims to identify manifestations of racism in the corporate environment and the disparities in the representation and advancement of black women, especially in the highest hierarchies of the institution. The justification for conducting this study lies in the gap in the literature on race and gender relations in the state of Goiás, particularly in the context of the labor market. Although Brazil has a large population of women in the banking sector, the presence of black women, especially in leadership positions, is significantly reduced. The research proves relevant to understand the dynamics of exclusion and the resistance strategies of these women in the corporate environment. The methodology adopted will be qualitative, with analysis of data obtained through semi-structured interviews with female employees of Banco Itaú, in addition to observations on the hierarchical structure and promotion criteria. The expected results include the identification of racial barriers and their social and psychological consequences, contributing to the reflection on more inclusive and anti-racist policies in the banking and corporate sector.
Edição gênica de Metarhizium Anisopliae Brm 2335 por Crispr/Cas9 “Marker-Free” e seu endofitismo em plantas de soja
(Universidade Federal de Goiás, 2023-03-12) Virgílio, Maria Letícia de Siqueira; Cortes, Marcio Vinicius de Carvalho Barros; Quintela, Eliane Dias; http://lattes.cnpq.br/7426637066408803; Cortes, Marcio Vinicius de Carvalho Barros; Filippi, Marta Cristina Corsi de; Quintela, Eliane Dias; Georg, Raphaela de Castro
The cosmopolitan fungus of the genus Metarhizium exhibits a versatile lifestyle, being effective in controlling various insect pests and also as a plant endophyte. The advent of CRISPR/Cas technology has revolutionized genome editing, offering simplicity, precision and cost-effectiveness. Although its application in entomopathogenic fungi has been limited, including M. anisopliae, recent advances show promising results. However, the integration of Cas9 and the selection of markers in fungal genomes present challenges, such as reducing their efficiency and toxicity and effects on non-target pests. Therefore, markerless genetic engineering through a recyclable CRISPR system presents a viable solution, allowing the efficient generation of mutants without compromising fitness and virulence. In the first chapter, this pioneering study constructed marker-free strains of M. anisopliae using a recyclable CRISPR/Cas9 system. The precise deletion of albA and ku70, together with the insertion of the gfp (green fluorescent protein) cassette confirmed the efficiency of the system. Furthermore, the M. anisopliae Δku70 and gfp constructs maintained their original fitness, killing adults of the brown soybean bug Euschistus heros with the same efficiency as the wild-type (WT) isolate. In the second chapter, the endophytic colonization of BRM 2335 in soybean plants after seed treatment was determined. Seed vigor and germination were not affected at doses of 1 × 108 and 5 × 108 conidia/mL. Seed treatment with 1× 108 conidia/mL significantly increased the dry mass of soybean plant roots only after seven days, but not after 14 days. On the other hand, the dry mass of the aerial part of the plants was similar for both untreated seeds and those treated with the fungus, at seven and 14 days. The length and height of soybean plants were also not affected by seed treatment with the fungus. In agar/water M. anisopliae was isolated in all stem samples and in all roots of the distal part. In soil, M. anisopliae was isolated endophytically in a few plant samples (14) and was found in the distal part of the root, stem, cotyledon and non-expanded primary leaves of soybean. Gfptagged Metarhizium facilitated the location and confirmation of isolate BRM 2335 in soybean plants. Isolation of BRM 2335 gfp in soybean plants was similar when seeds were treated with 1 × 108 , 5 × 108 and 1 × 109 conidia/mL. There was also no difference in the extraction of Metarhizium gfp by cutting part of the plants compared to macerating parts of the plants. In this study, endophytic colonization by Metarhizium was confirmed in a few samples of soybean plants, possibly due to competition from other fungi present in the soil. This innovative approach, CRISPR/Cas9, has great potential to facilitate in-depth molecular studies, understand their ecological roles in agricultural systems, and improve the effectiveness of biocontrol against insect pests through genetic improvements.