Mestrado em Ciência Animal (EVZ)
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Navegando Mestrado em Ciência Animal (EVZ) por Por Orientador "Carvalho, Rosângela de Oliveira Alves"
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Item Ação de um novo protótipo anti-hipertensivo (LASSBio897) sobre o sistema cardiovascular de cães(Universidade Federal de Goiás, 2014-02-26) Nasciutti, Priscilla Regina; Oliveira, Valéria de; http://lattes.cnpq.br/6300240031300604; Damasceno, Adilson Donizeti; http://lattes.cnpq.br/3900110295277130; Carvalho, Rosângela de Oliveira Alves; Carvalho, Rosângela de Oliveira Alves; Viana, Fernando Antonio Bretas; Lima, Aline Maria VasconcelosIn dogs, as in humans, arterial hypertension is characterized by an excessive increase of systemic blood pressure which can cause renal, ocular, neurological and cardiac lesions. Currently, five classes of antihypertensive agents are commonly used. A drug prototype (LASSBio 897) capable of promoting vasodilation effects was developed and became a possible alternative for new therapeutic strategy. The main goal of this study was to investigate its vasodilatory effect, as well as to identify possible changes in clinical, cardiovascular, renal and hepatic functions. Six adult and healthy beagle dog were used. They received oral doses of 0.5mg/kg, 1.0mg/kg and 2.0mg/kg of LASSBio 897 and 0.5mg/kg of benazepril, as a positive control. Assessments of liver and kidney function, doppler echocardiographic and electrocardiographic examinations and blood pressure measurements, were performed. No clinical changes suggesting acute toxicity were observed and all measured parameters were within the normal values for canine species. Blood pressure values decreased gradually until time of 120min, in which its lowest value was observed (120.58mmHg), among the three studied doses, similar to that observed with benazepril. No hypotension or arrhythmias were observed. In echocardiography, values of stroke volume and cardiac output were lower than those described as normal for the species, with averages that decreased until time of 240min. In conclusion the oral administration of the prototype LASSBio 897, in single doses, causes vasodilation and is safe since no significant changes in haematological, renal and hepatic biochemical evaluations were observed, as well as in urinary review. The prototype does not cause side effects, hypotension or abnormal heart rhythms.Item Ação de um novo protótipo anti-hipertensivo (LASSBio-897) sobre o sistema cardiovascular de gatos(Universidade Federal de Goiás, 2015-05-29) Santos Junior, Marcelo Borges dos; Oliveira, Valéria de; http://lattes.cnpq.br/6300240031300604; Fioravanti, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; Carvalho, Rosângela de Oliveira Alves; http://lattes.cnpq.br/5593911382353878; Alves, Rosângela de Oliveira; Tárraga, Kátia Mitsube; Damasceno, Adilson DonizetiSystemic hypertension (SH) is one of the main cardiovascular risk factors. It is a disease of often late detection due to its slow evolution and silence. Both, cats and man, SH is characterized by high and sustained levels of blood pressure (BP). The main consequences of SH are injuries in major organs, such as eyes, brain, kidneys, and heart. In order to avoid major problems generated by SH, researches have been conducted to identify new molecules capable to control SH. Recently a new prototype (LASSBio-897), was developed by the laboratory of Bioactive Substances (LASSBio) at the Federal University of Rio de Janeiro (UFRJ), which demonstrated significant vasodilation activity in rats. For a better understanding of these prototype, pre-clinical studies are needed in another specie, witch will serve to determine the pharmacokinetics and safety of the molecule. Therefore it is necessary, obeying the toxicological profile of the prototype, to pre-establish and administrate doses to another specie, in these case cats. In order to identify possible cardiovascular effects some complementary exams are needed, such as electrocardiogram, echodopplercardiogram and blood pressure measurement, besides it`s necessary to identify possible toxicity effects, so hematological exams are also needed, such as hemogram and serum biochemical. The aim of the study was to prove vasodilator effect induce by LASSBio-897 in cats. This effect was seen specially for the smallest dose of LASSBio-897 proposed.Item Parâmetros hemodinâmicos farmacocinéticos de ovinos submetidos anestesia com propofol(Universidade Federal de Goiás, 2017-07-31) Silva, Rogério Vieira da; Franco, Leandro Guimarães; http://lattes.cnpq.br/3331665967689811; Almeida, Ricardo Miyasaka de; http://lattes.cnpq.br/2593259109151413; Carvalho, Rosângela de Oliveira Alves; http://lattes.cnpq.br/5593911382353878; Carvalho, Rosângela de Oliveira Alves; Farias, Anderson; Damasceno, Adilson DonizetiSheep have been widely used as an experimental model for various anesthetic and surgical procedures, there is a shortage of information on the pharmacokinetics and hemodynamic behavior of anesthetic drugs in this species. No studies were found comparing the use of doppler echocardiography with a thermodilution in the measurement of the cardiac output of the animals. The objective of the study was to evaluate the pharmacological, hemodynamic and compare analyzes of the measurement of the cardiac output of sheep submitted to anesthesia with propofol. For this purpose, 10 healthy, uncastrated male sheep weighing 36 ± 4 kg on average were submitted to general anesthesia with propofol. One recommended induction dose to 6.5 mg / kg and the maintenance rate 0.5 mg / kg / minute. The protocol was determined by evaluating the loss of eyelid and laryngeal reflexes, allowing the accomplishment of endotracheal intubation. At time T0, T2, T5, T15, T30, T60, T65, T70, T75 were performed as collections of blood samples for pharmacokinetics through chromatography and evaluation of hemodynamic and respiratory parameters (HR, SBP, MAP, DAP). As well as measurements of cardiac output by Doppler echocardiography obtained at time T0, T5, T15, T30, T60. They were statistically evaluated by analysis of variance in a plot of subdivided plots without time, by Tukey test at 5% probability.The mean differences with the CO assessment methods were evaluated by the T test for paired data, along with an estimate of correlation between the data. A regression equation was also estimated to determine the values of the thermodilution technique as a function of the Doppler echocardiographic values. The evaluation of CO measurement methods, besides the comparison of the means obtained between the methods, evaluated the behavior of the values within the same technique over time. Plasma concentration of propofol showed a behavior in all animals during the evaluation period, generating its volume of concentration (56.2 ± 8.0 μg / mL) without non-T2 plasma (Tmax). The hemodynamic parameter was also observed as a reduction of blood pressure and PVR and of respiratory parameters and respiratory acidosis. It was observed that the CO values obtained by Doppler echocardiography remained always higher when compared to a Swan-Ganz catheter thermodilution. However, when the regression equation of the Doppler echocardiography values is applied as a function of thermodilution, very close values are obtained. Propofol induction without addition, but respiratory depression is identified by the increase in Paco2 values. Reduced blood pressure, as well as cardiac and systolic indexes are attributed to decreased peripheral vascular resistance. The Doppler echocardiographic method produces always higher values of CO when compared to those obtained by thermodilution, but it is possible to follow one direction of the changes in CO, in a reliable way, to use as two measurement techniques.Item Função sistólica do ventrículo esquerdo em cães com doença mixomatosa valvar mitral(Universidade Federal de Goiás, 2019-12-27) Sousa, Paulo Roberto de; Damasceno, Adilson Donizeti; http://lattes.cnpq.br/3900110295277130; Torres, Bruno Benetti Junta; http://lattes.cnpq.br/7060242425070126; Carvalho, Rosângela de Oliveira Alves; http://lattes.cnpq.br/5593911382353878; Carvalho, Rosângela de Oliveira Alves; Sousa, Marlos Gonçalves; Damasceno, Adilson DonizetiMyxomatous mitral valve disease (MMVD), or mitral valve endocardiosis, is the most prevalent acquired heart disease in dogs, being the main cause of heart failure in small dogs. The echocardiographic examination, which allows cardiac morphological and functional evaluation, is considered the “gold standard” test for diagnosing and staging MMVD in dogs. Information obtained by echocardiography on ventricular function also determines the prognosis in dogs with MMVD, since systolic dysfunction is a risk factor in these patients and contributes to the development of heart failure. However, the assessment of ventricular systolic function using conventional echocardiographic indexes is limited, since it is directly influenced by preload and afterload factors. The evaluation of cardiac mechanics by strain (ST) and strain rate (STR), using two-dimensional speckle tracking (2D-STE), allows, in humans, an earlier diagnosis of ventricular dysfunction. In addition, the mitral ring tissue displacement (TMAD) is an index of longitudinal systolic function that has a good correlation with conventional echocardiographic indices and low intra and interobserver variability. Thus, the objective of this study was to verify whether the myocardial deformity indexes (ST and STR) and the TMAD are more accurate and precocious than the conventional echocardiographic indexes in the assessment of left ventricular systolic function in dogs with MMVD, measuring the variables in the day of diagnosis (T0) and after 30 days (T30). For this purpose, 29 dogs were evaluated, categorized according to the ACVIM consensus, eight of which were in stage B1, 12 in stage B2 and nine in stage C. Considering the conventional variables used in this study, the presence of systolic dysfunction was not observed in dogs with MMVD, however changes after the start of pharmacological treatment were observed in the variables FET% and MAM. From the 2D-STE evaluation, similar to the conventional evaluation, systolic dysfunction cannot be determined in dogs with MMVD. However, the longitudinal ST variable indexed by weight showed a significant increase between the assessment times in group C, being still significantly higher in C than in B2, at administration, and higher in C than in the other groups after treatment. For the variable longitudinal STR it decreased in T30 in B1 and increased in C. When comparing the groups, in T30, group C showed higher values than in B1 and B2. Still, the variables longitudinal ST and TMAD showed a significant negative correlation when indexed to weight. In conclusion, the assessment of myocardial deformity using 2D-STE assessment did not demonstrate superiority compared to conventional indices for the diagnosis of systolic dysfunction in dogs with MMVD in its different stages.