Doutorado em Genética e Biologia Molecular (ICB)
URI Permanente para esta coleção
Navegar
Navegando Doutorado em Genética e Biologia Molecular (ICB) por Por Orientador "Amaral, André Corrêa"
Agora exibindo 1 - 2 de 2
Resultados por página
Opções de Ordenação
Item Desenvolvimento e caracterização de nanopartículas mistas de quitosana e lecitina contendo melatonina para o tratamento de feridas em ratos diabéticos(Universidade Federal de Goiás, 2020-08-19) Corrêa, Viviane Lopes Rocha; Leite, Liliana Borges de Menezes; http://lattes.cnpq.br/2012543423092393; Amaral, André Corrêa; http://lattes.cnpq.br/8801299423520104; Amaral, André Corrêa; Bocca, Anamélia Lorenzetti; Tedesco, Antônio Cláudio; Diniz, Danielle Guimarães Almeida; Miguel, Marina PachecoWound healing in diabetic patients remains a worldwide problem that can cause amputations and even lead to death. This work aimed to produce melatonin-loaded lecithin-chitosan nanoparticles (MEL-NP) and to evaluate a topical formulation containing these particles for healing in an in vivo animal model for diabetes. For the production of nanoparticles, an ethanolic solution containing soybean lecithin and melatonin was dropwise added to an aqueous solution of chitosan under sonication. The nanoparticles were physical-chemical characterized and evaluated in vivo for toxicity using the Galleria mellonella model and its potential for wound healing in diabetic rats. The MEL-NP were around 160 nm in size and had a zeta potential around 25 mV. The melatonin entrapment efficiency was 27%. Our results demonstrated that treatment with MEL-NP improved wound healing demonstrated by a wound closure earlier than the other treatments evaluated. An appreciated therapeutic effect was achieved by MEL-NP in the induction of fibroblasts and angiogenic proliferation. In addition, it was accompanied by an expressive collagen deposition. Taking the observed data, the MEL-NP produced could be used in the development of promising strategies for wound healing in diabetic people.Item Desenvolvimento e testes in vivo de nanopartículas de quitosana contendo insulina na cicatrização de feridas em ratos diabéticos(Universidade Federal de Goiás, 2019-04-16) Ribeiro, Maycon Carvalho; Menezes, Liliana Borges de; http://lattes.cnpq.br/2012543423092393; Amaral, André Corrêa; http://lattes.cnpq.br/8801299423520104; Menezes, Liliana Borges de; Miguel, Marina Pacheco; Mendes, Elizabeth Pereira; Souza, Taís Andrade Dias de; Silva, Luís Antônio Dantas daChitosan has been studied for its ability to accelerate healing and has been tested in the therapy of difficult-to-heal lesions, such as in diabetic patients. Insulin acts by stimulating the signaling pathway for wound healing. The objective of this work was to produce chitosan nanoparticles containing insulin for the evaluation of cicatrizant activity in diabetic rats. For the formation of the nanoparticles, the ionic gelation method was used. The nanoparticles were analyzed by diameter, potential zeta polydispersity index. The degree of deacetylation of chitosan by potentiometric was determined. For the insulin- associated nanoparticles, the mean diameter was 245.9 ± 25.46 nm and zeta potential of 39.3 ± 4.88 mV and PDI of 0.463 ± 0.01. The mean degree of deacetylation found was 72.95%. The Bradford assay revealed that the nanoparticles incorporated 97.19% ± 2.18 of insulin. To evaluate the healing, 72 Wistar rats were divided in four groups: sepigel (S), sepigel with insulin (SI), empty chitosan nanoparticles (QV) and chitosan nanoparticles containing insulin (IQ). The groups were subdivided into three subgroups (n = 6) according to the histological analysis times of the wound (3rd, 7th and 14th day). The induction of diabetes occurred through the intraperitoneal application of alloxan (120mg / kg). After confirmation of the diabetes state, the animals were anesthetized and the wounds were made with an 8.0 mm punch in the dorsal region. Macroscopic and microscopic analyzes were performed. It was possible to produce chitosan nanoparticles by the ionic gelation method, with desired diameter and zeta potential and polydispersity index. No differences were found in the rate of wound retraction among the four groups. The topical use of empty or insulin-containing chitosan nanoparticles in wound healing in diabetic rats was able to stimulate inflammatory cell proliferation and angiogenesis, followed by wound maturation. Differences in wound healing data from the group treated with insulin-containing nanoparticles and from the group treated with free insulin may be related to the high stability of the