Doutorado em Genética e Biologia Molecular (ICB)
URI Permanente para esta coleção
Navegar
Navegando Doutorado em Genética e Biologia Molecular (ICB) por Por Orientador "Soares, Célia Maria de Almeida"
Agora exibindo 1 - 4 de 4
Resultados por página
Opções de Ordenação
Item Metabolismo do acetato entre membros do gênero paracoccidioides spp(Universidade Federal de Goiás, 2017-04-13) Mata, Fabiana Ribeiro da; Soares, Célia Maria de Almeida; http://lattes.cnpq.br/8539946335852637; Soares, Célia Maria de Almeida; Ricart, Carlos André Ornelas; Ulhoa, Cirano José; Bailão, Alexandre Melo; Brito, Wesley de AlmeidaMembers of the genus Paracoccidioides are known pathogens of humans that can be isolated from different infection sites. To investigate the expression rates of proteins expressed by different isolates, of the genus Paracoccidioides, including one isolate of P. lutzii (Pb01), and three isolates of P. brasiliensis (Pb03, Pb339 and PbEPM83) using sodium acetate, as carbon source, proteins quantities were determined using label-free and data independent LC-MSE. Statistical analysis provided the comparison of proteins profiles in the isolates. A total of 1160, 1211, 1280 and 1462 proteins were reproducibly identified, and relatively quantified in P. lutzii e P. brasiliensis isolates Pb03, Pb339 e PbEPM83, respectively. Notably, a total of 526, 435, 744 and 747 proteins were differentially expressed among P. lutzii and the P. brasiliensis isolates Pb03, Pb339 and PbEPM83, respectively with a fold change equal or higher than 1.5. The analysis revealed the reorganization of metabolism through the induction of proteins related to gluconeogenesis, stress response and amino acid degradation in the four isolates evaluated. The differences between the isolates were observed as follows: greater increases in the expression levels of proteins belonging to the cycle of glyoxalate, TCA and respiratory chain, ethanol production and β-oxidation were observed in the isolatesPPEPM83 and Pb01; Cyclic and cyclocyclic proteins of the methylcitrate were induced in Pb339. Proteomic profiles indicate that the four isolates reorganize the metabolism for the use of acetate as a carbon source.Item Análise proteômica do fungo Paracoccidioides brasiliensis sob privação de cobre(Universidade Federal de Goiás, 2020-04-27) Petito, Guilherme; Soares, Célia Maria de Almeida; http://lattes.cnpq.br/8539946335852637; Soares, Célia Maria de Almeida; Castro, Agenor de; Silva, Lívia do Carmo; Curcio, Juliana Santana de; Silva, Kleber Santiago Freitas eCopper is an important micronutrient that acts as a cofactor in important enzymes that participate in processes of obtaining energy, reducing reactive oxygen species and metal uptake such as iron. Studies with different pathogenic fungi demonstrate the importance of an efficient copper homeostasis system, as well as the impact of this metal deprivation on the metabolism of these organisms. However, little is known about the impact of copper deprivation on pathogenic fungi of the genus Paracoccidioides. Thus, the objective of this study was to analyze the proteomic behavior of the fungus Paracoccidioides brasiliensis during copper deprivation, identifying metabolic changes in face of this condition. Proteomic analysis was performed using mass spectrometry and labelling the samples with iTRAQ (Isobariq tag for relative and absolute quantitation). In addition, methodologies such as qRT-PCR, fluorescence microscopy, enzymatic activities and Western bloting were applied in this study in order to support the proteomic data analyzed. We identified 183 differentially expressed proteins. Proteins related to gluconeogenesis, beta-oxidation and cell wall remodeling were up-regulated. We identified decreased expression of an important detoxification enzyme, Cu / Zn dependent Superoxide Dismutase (Sod1p). On the other hand, the enzymes Thioredoxin (Trxp), Glutathione S-transferase (GST) and Mn / Fedependent superoxide dismutase (Sod2p) were up-regulated. Also, NADPH production-related to the pentose-phosphate pathway enzymes. detoxifying enzyme reducing agent. An alternative oxidase (Aoxp) was up-regulated, while enzyme activity tests showed that the activity of the enzyme Cytochrome c oxidase (Coxp) was decreased. An iron uptake-related copper chaperone, Atx1 and described as important in response to oxidative stress, was up-regulated. We propose here an overview that presents the main metabolic changes in P. brasiliensis under copper deprivation and that include changes in the energy acquisition process, carbon metabolism flow, wall modeling, detoxification and respiration processes.Item Análise proteômica comparativa entre Paracoccidioides brasiliensis e Paracoccidioides americana(Universidade Federal de Goiás, 2019-12-19) Portis, Igor Godinho; Ricart, Carlos André Ornelas; http://lattes.cnpq.br/1271167329803156; Soares, Célia Maria de Almeida; http://lattes.cnpq.br/8539946335852637; Soares, Célia Maria de Almeida; Lima, Patrícia de Sousa; Curcio, Juliana Santana De; Pereira, Maristela; Rocha, Juliana Alves ParenteThe genus Paracoccidioides comprises termo dimorphic fungi that cause paracoccidioidomycosis, the most prevalent systemic mycosis in Latin America. These fungi can grow in yeast at 36 ° C and mycelium at temperatures below 28 ° C. Studies have shown that the fungus Paracoccidioides has molecular differences that allow the classification of species. Initially those fungi were classified in a single species, Paracoccidioides brasiliensis, but with the advancement of some molecular and genomic biology techniques, Paracoccidioides is currently classified into five species, P. lutzii, P. brasiliensis, P. americana, P. restrepiensis and P. venezuelensis. However, different species may have different proteomes and cellular responses. In this way in our study, we performed proteomic analyzes of two species of Paracoccidioides, P. americana and P. brasiliensis. iTRAQ labelling and mass spectrometery allowed the identification of 387 statistically significant proteins; two hundreds and two in P. brasiliensis and 111 proteins in P. americana. The identified proteins have different functions, such as amino acid metabolism, energy, cell defense, among others. The results showed differences in protein responses between P. americana and P. brasiliensis in proteins related to cell defense, mitochondria and energy metabolism, suggesting that these fungi may have different metabolism and pathways for growth, profliferation and virulence.Item Homeostase de ferro em Paracoccidioides spp.: novos alvos de estudo e estabelecimento de HSP30 como proteína ligante de hemoglobina(Universidade Federal de Goiás, 2021-06-28) Souza, Aparecido Ferreira de; Soares, Célia Maria de Almeida; http://lattes.cnpq.br/8539946335852637; Soares, Célia Maria de Almeida; Schrank, Augusto; Parente, Ana Flávia Alves; Bailão, Elisa Flávia Luiz Cardoso; Bailão, Alexandre MeloUnderstanding the mechanisms that govern host-pathogen interaction is crucial for the development of new therapeutic and diagnostic approaches. Fungi of Paracoccidioides genus are the etiological agents of paracoccidioidomycosis, a systemic mycosis and, when in the host, fungi find a hostile environment that is scarce in nutrients and micronutrients, such as Fe, which is indispensable for the survival of the pathogen. Previous studies have shown that fungi of this genus, in response to Fe deprivation, are able to synthesize and capture siderophores (Fe3+ chelators), use host proteins that contain Fe as metal’s source and use a non-canonical reductive pathway for assimilation of Fe. Despite all these findings, there are still gaps that need to be filled regarding the pathogen's response to Fe deprivation. In the present work, targets that still need to be studied in this context were defined, such as the establishment of the protein functions of the fungus that contain the CFEM domain and the determination of the specificity of siderophore receptors. Additionally, it was shown that the exposure of Paracoccidioides to hemoglobin promotes changes at fungal cell wall proteome level, which is illustrated by the increased ability of the fungus to interact with macrophages. This approach allowed the bioprospecting of the hemoglobin-binding protein HSP30, which is present on the cell surface and has its expression regulated positively when the fungus is exposed to hemoglobin. It has been shown that silencing of hsp30 causes decreased growth of the fungus after exposure to hemoglobin. It is also necessary to investigate the function of HSP30 as heme oxygenase. The detailed observation of those open questions can promote the expansion of knowledge about the biology of Paracoccidioides spp. and, consequently, promote the bioprospecting of new targets for differential diagnosis and/or therapy of PCM.