Programa de Pós-graduação em Ciências Fisiológicas - Multicêntrico
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Navegando Programa de Pós-graduação em Ciências Fisiológicas - Multicêntrico por Por Orientador "Colugnati, Diego Basile"
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Item Avaliação das respostas cardiovasculares e eletroencefalográficas após crises epilépticas induzidas pelo abrasamento elétrico da amígdala em diferentes fases do ciclo sono-vigília de ratos(Universidade Federal de Goiás, 2016-09-21) Ghazale, Poliana Peres; Pansani, Aline Priscila; http://lattes.cnpq.br/6385679829734771; Colugnati, Diego Basile; http://lattes.cnpq.br/3875833705952056; Colugnati, Diego Basile; Blanch, Graziela Torres; Oliveira, André Henrique Freiria; Pansani, Aline PriscilaIntroduction: Cardiovascular alterations have been identified as the main cause of Sudden Unexpected Death in Epilepsy (SUDEP). There is an intimate relationship between epilepsy and sleep. Most cases of SUDEP occurs during sleep and the NREM sleep is a facilitator factor for the occurence of seizures. Aim: To evaluate cardiovascular and electroencephalographic responses of rats submitted to kindling model induced at diferente stages of sleep-wake cycle. Methods: The experimental protocol was approved by Ethics Commitee of the Universidade Federal de Goiás (CEUA-035/2015). Wistar rats (250-350 g) were separeted into 4 groups and seizures were induced according to sleep-wake cycle (Wake, NREM sleep and REM sleep) or no seizure (SHAM). We used the kindling model for induction of seizures. The animals was submitted to stereotactic surgery to implant electrodes in the right amygdala, for electrical stimulation, and in the cortex, for electroencephalographic record (EEGc). Electrodes for electromyographic (EMG) and electrocardiographic (ECG) recordings were also implanted. The detection of sleep phase was based on algorithms described by Louis et al. (2003). The electrical stimulus was induced one time per day and consisted in a biphasic wave during 2 seconds (60 Hz). The seizures evolution was analyzed according to Racine’s scale (E1: orofacial movements; E2: mastigatory movements and head clonus; E3: forelimb clonus; E4: rearing; E5: rearing and falling). Three E5 seizures was induced in each animal. We evaluated heart rate (HR), heart rate variability (HRV) arrhythmias and EEGc in vivo, cardiac function and vascular reactivity ex vivo. Results: The time fully kindling in NREM group was lower compared to Wake group. The NREM group presents postictal tachycardia in the early stages of process. The duration of postictal generalized electroencephalogram supression (PGES) is larger in REM group. The PGES in Wake group was accompanied by a decrease in HR compared to the final period of seizures. The muscle cell in aortic artery of Wake group had greater contraction capacity than the other groups, while the REM group had a higher endothelium-dependent relaxatio. Parameters such as after-discharge duration (AD), HR and HRV after kindling process, arrhythmias, bradycardia followed by tachycardia during the ictal period of E5 seizures and ex vivo cardiac funtion were similar in all groups. Conclusion: Our results suggest that kindling process per se was able to induce an increase in post-ictal HR in NREM group, an increase in PGES duration in REM group, and an impairment on vascular reactivity of NREM (higher contraction percentage) and REM (lower relaxation percentage) groups.Item Avaliação de alterações cardiovasculares relacionadas ao efeito de drogas antiepilépticas em ratos submetidos ao modelo de indução de epilepsia pela pilocarpina(Universidade Federal de Goiás, 2018-05-18) Souza, Beatriz Pacheco de; Colugnati, Diego Basile; http://lattes.cnpq.br/3875833705952056; Lino Júnior, Ruy de Souza; Castro, Carlos Henrique de; Colugnati, Diego BasileEpilepsy is one of the most common problems in the neurological clinic, affecting up to 1% of the world population, moreover, individuals with epilepsy have a higher mortality than the general population. Thus, the interest in investigating cardiac changes in patients with epilepsy has been increasing, and some studies have associated the use of antiepileptic drugs (AEDs) with cardiovascular disorders. In this context, there are hypotheses that patients using specific AEDs, sodium channel blockers, have an increased risk of cardiovascular disease (CVD). In our study, we sought to evaluate cardiovascular responses in epileptic rats submitted to chronic administration of carbamazepine (CBZ) or lamotrigine (LTG). Baseline cardiovascular parameters [Systolic blood pressure (SBP); Diastolic blood pressure (DBP); Mean arterial pressure (MAP) and heart rate (HR)] were recorded through cannulation of the femoral artery and electrode implant after sixty days of treatment with AED or vehicle [(150mg / kg (vo)]. The animals were submitted to a baroreflex test with bolus administration of phenylephrine (PHE - 5μg) and sodium nitroprusside (NPS - 10μg) via cannulation of the femoral vein and later submitted to a challenge with isoproterenol. Through the recording of baseline cardiovascular parameters, we also analyzed the heart rate variability (HRV) and the number of extrasystoles. After all procedures in vivo, samples of the heart were retained for histological analyzes of cardiac tissue. The division of the groups was performed in controls without epilepsy (CNT), epileptics (EP), epileptics treated with CBZ (CBZ) and epileptics treated with LTG (LTG). In our results the epileptic rats presented all the baseline cardiovascular parameters higher than the parameters of animals without epilepsy. CBZ treatment reduced resting HR, SBP and MAP compared to untreated epileptic animals. Treatment with LTG also reduced resting HR compared to the EP group. We also observed that the EP group had a greater cross-sectional area (CSA) of cardiomyocytes when compared to the other groups and an increased accumulation of perivascular collagen in comparison to CNT and CBZ groups. In this way, we can suggest that chronic use of AEDs may influence cardiovascular responses and cardiac microstructure.Item Avaliação da reatividade coronariana do coração isolado de ratos submetidos ao modelo de indução de epilepsia pela pilocarpina(Universidade Federal de Goiás, 2016-09-19) Vitorino, Paula Rodrigues; Pedrino, Gustavo Rodrigues; http://lattes.cnpq.br/1155446449250341; Colugnati, Diego Basile; http://lattes.cnpq.br/3875833705952056; Magalhães, Fernando Henrique; Pansani, Aline Priscila; Colugnati, Diego BasileEpilepsy is the most common chronic neurological disease in the world, characterized by paroxysmal, excessive and synchronous discharges of a neuronal population that leads to spontaneous and recurrent seizures. Sudden unexpected death in epilepsy (SUDEP) is responsible for 7.5% to 17% of deaths in epilepsy. Although the pathophysiological mechanisms are unknown, one possible explanation is that they are of cardiogenic origin. Some studies relate cardiac abnormalities with seizures, and these may be responsible for Suped, and as yet has no works that have investigated the control of coronary flow of patients or in experimental models of epilepsy, and as coronary heart disease listed as one of the main causes of sudden death in the world population, assess coronary flow in experimental epilepsy model becomes important for the understanding of SUDEP. So this study aims to evaluate the coronary reactivity, ventricular function and cardiac tissue of rats submitted to the pilocarpine model of epilepsy. The animals were separated into two groups: control (n = 8) and epilepsy (n = 8). It was administered 350 mg/kg of pilocarpine (i.p) preceded by 1mg/kg methylscopolamine (s.c) in both groups, animals that entered in status epilepticus received diazepam 10 mg/kg (i.p) after 3 hours to block it. After that, the animals were placed in a room for video monitoring (24 h/day) until complete two months of epilepsy (epilepsy group). Rats that received pilocarpine and did not develop status epilepticus comprised the control group, being housed in the same animal environment that epilepsy group. At two months of chronic epilepsy rats were sacrificed and the heart dissected to the Langendorff preparation (constant flow), after a 35 minutes of stabilization in a Krebs - Ringer solution bradykinin (BK) was administered (10ˉ⁸, 10ˉ⁷, 10ˉ⁶ and 10ˉ⁵M) in bolus and after of washout was treated with sodium nitroprusside in different concentrations (10ˉ⁶, 10ˉ⁵, 10ˉ⁴ and 10ˉ³M) also bolus. They were found in animals with epilepsy a significant reduction in coronary relaxation by BK infusion at a concentration 10ˉ⁵M. It was observed that rats with epilepsy have increased perivascular collagen, larger cardiomyocytes, and more coronary arteries, with no change of nitric oxide synthase endoletial (eNOS), superoxide dismutase (SOD) and catalase expression. Thus, our results show reduction of coronary relaxation induced by bradykinin which leads us to believe in loss of endothelial function of these animals, since, we did not observe differences in relaxation induced by sodium nitroprusside, despite the perivascular fibrosis of epileptic rats.