Doutorado em Odontologia (FO)
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Navegando Doutorado em Odontologia (FO) por Por Orientador "Estrela, Carlos"
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Item Efeito da terapia fotodinâmica sobre biofilme de Enterococcus faecalis e estrutura dentinária(Universidade Federal de Goiás, 2016-05-05) Alves, Denise Ramos Silveira; Alencar, Ana Helena Gonçalves de; http://lattes.cnpq.br/2039898015468283; Estrela, Carlos; http://lattes.cnpq.br/3611967334176683; Estrela, Carlos; Souza, João Batista de; Decúrcio, Daniel de Almeida; Pécora, Jesus Djalma; Barletta, Fernando BrancoObjective: Evaluate the effect of photodynamic therapy (PDT) on Enterococcus faecalis biofilm in infected root canals and on dentin structure. Methods: Twenty-one root canals of a sample of 24 extracted single-rooted human teeth were infected by E. faecalis for 60 days to form biofilm. The antimicrobial strategies tested were (n=3 in each group): root canal preparation using NiTi rotary instruments, 2.5% NaOCl and 17% EDTA irrigation, and PDT with 0.01% methylene blue (group I) or 0.01% malachite green (group II); root canal preparation using NiTi rotary instruments, 2.5% NaOCl and 17% EDTA irrigation, and PDT with 0.01% methylene blue (group III); PDT with 0.01% methylene blue without root canal preparation (group IV); root canal preparation using NiTi rotary instruments, 22.5% NaOCl and 17% EDTA irrigation, and no PDT (group V); 2.5% NaOCl irrigation with no root canal preparation, and 17% EDTA irrigation (group VI); positive control (group VII). Three roots were not infected and were used as negative controls (group VIII). Samples for microbiological tests were collected using three sterile paper points, later stored in BHI and incubated at 37o C for 48 hours at three time points: before (S1) and after (S2) root canal preparation, and after PDT application (S3). Bacterial growth was analyzed according to turbidity of culture medium, presence of bacteria, and spectrophotometric optical density (nm). Specimens were sectioned and prepared for SEM analysis of dentin structure. Results: Bacteria were found at S1, S2 and S3 in all experimental groups. Optical density of culture media at S2 and S3 in groups I, II and III were lower than at S1, but not statistically different. Optical density of culture media at S2 was 28.70% and 24.67% lower than at S1 in groups I and II; after PDT, optical density was 90.00% (group I) and 37.30% (group II) lower. In group III, it was 97.70% lower at S2 and an additional 92.00% lower after PDT. In group IV, optical density increase 3.2%. Dentin analysis after PDT revealed areas of melting and recrystallization, peritubular dentin projections, intertubular dentin erosion and fusion of dentinal tubule openings, which made dentin surface irregular. Some dentinal tubules were obliterated, and there were changes in the shape of their openings. Conclusion: PDT applied after root canal preparation using manual or rotary files was not effective in eliminating E. faecalis completely. PDT changed dentin structure and resulted in dentin melting and recrystallization, as well as in dentinal tubule erosion and obliteration.Item Avaliação de cimentos e técnicas de obturação no preenchimento de istmos radiculares usando um novo software de tomografia computadorizada de feixe cônico(Universidade Federal de Goiás, 2022-05-25) Morais, André Luiz Gomide de; Estrela, Carlos; http://lattes.cnpq.br/3611967334176683; Oliveira, Helder Fernandes de; Siqueira, Patrícia Correia de; Silva, Júlio Almeida; Silva, Brunno Santos de Freitas; Estrela, CarlosAim: To evaluate the sealing ability of root isthmuses using different sealers and root canal filling techniques using a new Cone Beam Computed Tomography software (e-Vol DX). Methodology: 120 lower molars extracted from patients with indication for extraction were used. The teeth were divided into 6 groups according to the endodontic sealer and filling technique: G1: AH Plus® + lateral condensation technique (n=20), G2: AH Plus® + Tagger's hybrid technique (n=20), G3: AH Plus® + single cone technique (n=20), G4: BioRoot RCS + lateral condensation technique (n=20), G5: BioRoot RCS® + Tagger hybrid technique (n=20) and G7: BioRoot RCS® + single cone technique (n=20). The teeth were instrumented using the Wave One Gold® reciprocating system, irrigation performed with 2.5% sodium hypochlorite and EDTA (using ultrasonic passive irrigation) and then all canals were filled according to the division of the groups. Sealing evaluation was performed using scores. Nonparametric statistics were used for its analysis and the description was performed by median, minimum and maximum. Mann Whitney test was used to compare the scores between the sealers and Kruskal-Wallis test followed by Dunn-Bonferroni post hoc tests between the obturation techniques and groups. To compare scores obtained with and without Blooming Artifact Reduction (BAR) filter of e-Vol DX software, Wilcoxon test was used. Results: Results showed there was a significant difference in sealing lower molar isthmus using endodontic sealers studied in apical third with BAR (p=0.016), with scores being higher for AH Plus®. There were differences in sealing of lower molar isthmus using different filling techniques in middle and apical thirds. For middle third without BAR, difference was between single cone and Tagger's hybrid techniques (p=0.011) as well as for apical third without BAR (p<0.001). For middle third with BAR, difference was between single cone technique and lateral condensation (p=0.043) and Tagger hybrid (p=0.009) techniques. For apical third with BAR, the difference was between Tagger's hybrid technique and single cone (p<0.001) and lateral condensation (p=0.041) techniques. When comparing the sealing scores using different filling techniques and types of sealer, there were differences between G2 and G3 (p=0.015) and G2 and G6 (p=0.024) in apical third without BAR. Using BAR tool, statistical differences were as follows: in cervical third between G2 and G6 (p=0.023); in middle third, between G2 and G6 (p=0.072); in apical third, G6 had lower scores than G1 (p=0.046), G2 (p=0.002) and G5 (p=0.011) and G4 had lower scores than G2 (p=0.023). When comparing isthmus sealing scores with and without using BAR tool, there were significant differences, with higher values without using the tool, in all thirds. It is concluded that, when evaluating sealing ability of root isthmus using different sealers and filling techniques with e-Vol DX software, the best combination was the use of AH Plus and Tagger's hybrid technique. Using BAR filter of the eVol DX software in CBCT images showed more empty spaces in filling mass due to the reduction of white expansion, making tomographic image more reliable.