Mestrado em Ciências Farmacêuticas (FF)
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Navegando Mestrado em Ciências Farmacêuticas (FF) por Por Orientador "BOZINIS, Marize Campos Valadares"
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Item Investigação da atividade antitumoral in vitro e in vivo da Eugenia dysenterica DC. (MYRTACEAE)(Universidade Federal de Goiás, 2011-08-30) ANDRADE, Wanessa Machado; PAULA, José Realino de; http://lattes.cnpq.br/3191837532986128; BOZINIS, Marize Campos Valadares; http://lattes.cnpq.br/6157755243167018Item Investigação da mielotoxicidade e do potencial indutor de morte celular de Synadenium umbellatum Pax. em células do tumor ascítico de Ehrlich(Universidade Federal de Goiás, 2010-03-03) MOTA, Mariana Flávia da; BOZINIS, Marize Campos Valadares; http://lattes.cnpq.br/6157755243167018In search of new antineoplasic agents natural products have played an important role, given that about 40% of available medicines in current treatments have been developed from natural sources, 25% approximately of plants. Synadenium umbellatum belongs to Euphorbiaceae family and is popularly known as "cola-nota", "avelós," "milagrosa", "cancerola", and the latex is empirically used in Brazil for the treatment of cancer. Preliminary studies have shown that the crude extract of the leaves of S. umbellatum has significant antitumor and antiangiogenic activity in vivo. In this study, we investigated the cytotoxicity and the mechanisms of apoptosis induction of S. umbellatum in Ehrlich ascitic tumor (EAT) cells, and the myelotoxic potential of this plant. The cytotoxicity studies were carried out by trypan blue exclusion and reduction of tetrazolium (MTT) tests. The mechanisms of apoptosis induction were investigated by light and fluorescence microscopy, immunocytochemistry and flow cytometry. Investigation of the myelotoxic potential was performed by clonogenic assay of colony forming units of granulocyte-macrophage (CFU-GM). The data were analyzed by analysis of variance (ANOVA) and a posteriori Tukey test (myelotoxicity) and by t test for all other assays. The means were considered statistically significant when P < 0.05. S. umbellatum was cytotoxic to EAT cells and the morphological analysis of these cells indicated that the plant extract treatment induced cell death by apoptosis. Apoptosis was also observed by increase on reactive oxygen species generation and in the concentration of intracellular Ca2+, alterations in mitochondrial membrane potential, phosphatydylserine externalization and activation of caspases 3, 8, and 9. S. umbellatum presented myelotoxicity to normal bone marrow cells in a concentrationdependent fashion. However, this toxicity was 8-fold lower than to tumor cells. The presented data showed that S. umbellatum had cytotoxic activity to EAT cells and induced these cells to apoptosis.Item INVESTIGAÇÃO DO POTENCIAL TÓXICO DO EXTRATO BRUTO ETANÓLICO DAS SEMENTES DE ANNONA CORIACEA MART. (ARATICUM) EM CAMUNDONGOS SUBCRONICAMENTE EXPOSTOS(Universidade Federal de Goiás, 2008-09-25) NASCIMENTO, Guilherme Nobre Lima do; FERREIRA, Tales Alexandre Aversi; http://lattes.cnpq.br/1101341437463729; BOZINIS, Marize Campos Valadares; http://lattes.cnpq.br/6157755243167018The araticum (Annona coriacea Mart.) is a typical fruit of the brazilian cerrado used popularly to overcome inflammatory processes. The family Annonaceae presents as the main constituents acetogenins, a class of substances with great cytotoxic and genotoxic potential, and cited as responsible for a disease similar to parkinson in a Caribbean population that used the fruit as much as food and for medicinal purposes. The aim of this study was to evaluate the putative activity of crude ethanolic extract of seeds of A. coriacea on the cerebral cortex of mice exposed at doses of 12.5, 25, 50 and 100 mg / kg, and evaluate its activity on different areas of the brain, the liver and kidneys. We used 30 adult male Swiss mice divided into groups control, solvent and treated (12.5, 25, 50 and 100 mg / kg). The extract was administered orally for four days. The target organs were extirpated, fixed in 70% ethanol (v / v) and processed for histological method - hematoxylin and eosin. The analysis of the slides was performed by image processing system for counting cells and other morphometric analysis. The morphological studies showed no significant changes to the brain in different areas, just as no changes were detected in the kidneys. On the other hand, was found a reduction on the frequency of cells per area of the liver, like as an reduction on the consumption of food, water and production of excreta. We conclude with this work a possible hepatotoxic activity induced by exposure to crude ethanol extract of seeds of A. Coriacea Mart., observed by the decrease in frequency of cells per area of the liver, correlated with reductions in consumption of food / water and production of excreta by animals.Item Atividade antitumoral e antiangiogênica de Synadenium umbellatum Pax in vitro e in vivo(Universidade Federal de Goiás, 2008-03-11) NOGUEIRA, Iara Antonia Lustosa; BOZINIS, Marize Campos Valadares; http://lattes.cnpq.br/6157755243167018Synadenium umbellatum Pax is a vegetable belonging to Euphorbiaceae, popularly known as cola-nota , milagrosa , and cancerola , is empirically used as a natural remedy for fighting cancer. In this study we assessed the antitumoral and antiangiogenic activity in vitro and in vivo of ethanolic crude extract and hexanic and chloroformic fractions of S. umbellatum. Citotoxicity assessment tests in vitro were carried out through MTT reduction and Trypan blue exclusion methods, by using Ehrlich ascitic tumor cells and K-562 lineage. Antitumoral and antiangiogenic activity in vivo essays were carried out through Ehrlich ascitic tumor experimental model in Swiss mice. Ethanolic crude extract was tested in 5, 10 and 25 mg/kg/day doses, whereas chloroformic fraction was tested in 1.2; 2.3 and 5.8 mg/kg/day doses, and hexanic fraction in 0.4; 0.8; and 1.9 mg/kg/day doses, for 10 days. The results showed concentration-dependent citotoxic activity, presenting through MTT method the inhibitory concentrations of 50% (Cl 50) 0.4, 0.1, and 0.08 mg/ml for ethanolic crude extract, chloroformic and hexanic fractions, respectively. And in the Trypan blue exclusion test, cell inhibition was 75%, 68%, and 80%, and 95%, 58%, and 64% for Ehrlich ascitic tumor cells and K-562 lineage, respectively. Ethanolic crude extract in the 25mg/kg/day dose increased the animals survival rate, parallely with the reduction of the amount of tumoral cells, beyond present activity on the tumor angiogenesis, and reduce vascular endothelial growth factor levels. Chloroformic and hexanic fractions, in in vivo essays, did not present significant outcomes. Thus, can conclude in the condition this study, that S. umbellatum shows antitumoral and antiangiogenic activity