Mestrado em Ciência Animal (EVZ)
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Navegando Mestrado em Ciência Animal (EVZ) por Por Unidade Acadêmica "Ciências Agrárias"
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Item Anestesia intravenosa total com cetamina-propofol ou cetamina-xilazina-egg em infusão contínua em asininos pré-medicados com xilazina(Universidade Federal de Goiás, 2009-06-26) COELHO, Cássia Maria Molinaro; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; ALVES, Geraldo Eleno Silveira; http://lattes.cnpq.br/2315947597632104; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647The dissociative anesthesia was receiving more attention as the inhalation anesthesia based in cardiorespiratory and economic results in horses. However, there are few studies in donkeys (Equus asinus). The aim of this research was evaluate and compare the cardiorespiratory, metabolic and electrocardiographic effects of ketamine-propofol (KP) and ketamine-xilazine-EGG (GKX) total intravenous anesthesia in donkeys. Eight healthy adult donkeys were anesthetized twice with seven days interval between protocols. In both protocols, the animals were premedicated with 1.0 mg/kg of xylazine. The protocol KP was induced with 1.5 mg/kg of ketamine and a loading dose of propofol (0.5 mg/kg). The animals were maintained in constant rate infusion of ketamine (0.05 mg/kg/min) and propofol (0.15 mg/kg/min) during one hour. In GKX protocol, the animals were induced with diazepam (0.05 mg/kg) and ketamine (2.2 mg/kg). So, the animals were maintained in constant rate infusion (2 ml/kg/h) of a solution prepared with an association of 5% guaifenesin (100 mg/kg), ketamine (2.0 mg/ml) and xylazine (0.5 mg/ml). The quality of induction and recovery were assessed using scores (1-good; 2-regular; 3-poor) and measured cardiorespiratory, metabolic and electrocardiographic parameters before, during and after the anesthetic procedure. The results showed that the ketamine-propofol and ketamine-xylazine-EGG associations were satisfactory to anesthetized the donkeys, whereas seven anesthetic inductions were classified as good (score 1) in both protocols. The heart rate was statistic different between protocols since 15M moment. The mean arterial pressure decreased during the procedure in KP and GKX. There were no statistic differences between protocols in PaO2, PaCO2, LACv e LACa. The arterial glucose showed higher elevation in GKX and statistic difference at recovery. There were no statistic differences regarding CK and AST serum values. Concerning the electrocardiographic assessment, the protocols did not result in abnormalities on the length and amplitude of waves and complexes that could compromise the cardiac dynamics. In conclusion, propofol-ketamine and ketamine-xylazine-EGG associations can be successfully employed in asinines for the accomplishment of procedures without painful stimuli. The cardiorespiratory, metabolic and electrocardiographic changes observed in the present study were not sufficient to cause systemic distress in healthy animalsItem Detecção de resíduos de soluções sanitizantes empregadas em pedilúvio para bovinos no leite e solo(Universidade Federal de Goiás, 2011-07-04) GOULART, Daniel Silva; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647Copper sulfate and sodium hypochlorite are used in the preparation of cattle footbath solutions, aimed at treating and preventing digital diseases, but little is known about the residues that these substances leave in animal products. The purpose of this study was to evaluate, on soil and milk, residues left by chemicals used in cattle foot bath. The study was developed in two stages. In the first stage, the residual of chlorine and copper in the milk of healthy cows after successive passages in footbath solutions containing these substances was analyzed and the costs of the solutions were estimated. The presence of copper residues was evaluated using atomic absorption spectrophotometry and chlorine residues were evaluated by dosing total chloride in milk using a commercial kit. In the experiment, 14 healthy cows were divided into two groups (GI and GII) of seven animals each. In GI a solution of sodium hypochlorite 1% was used and in GII copper sulphate 5% was used. The milk samples were collected before the passage through the footbath (M0) after 24 (M1), 48 (M2) and 72 hours (M3) and 15 days (M15), following the last passage. Statistical analysis comparing the moments of each sample and the substances evaluated was performed using Friedman test followed by Dunn's test (p <0.05). It was concluded that the values of total chloride and copper in the milk of healthy cattle, after daily passage in footbath for a period of seven days, showed some variations considered insufficient to cause harm to human health and the solutions did not show exorbitant costs. In the second stage, copper residues were evaluated in soil treated with a mixture of cattle feces and urine, simulating cattle liquid manure, containing or not copper sulfate solution. Initially samples were collected from soil in pastures of EVZ / UFG, which were placed in PVC columns divided into four layers. The columns were divided into three treatments (CI, CII and CIII). The CI treatment received only cattle liquid manure and the CII and CIII treatments received the same material added of different amounts of copper sulfate solution. During 120 days, rain was simulated on the PVC columns. In the statistical analysis, analysis of variance followed by the Scott-Knott test at 5% probability were used. It was observed that copper levels were significantly higher in CIII treatment than in CI and CII. The same is true regarding the treatments CII for CI. In treatments CII and CIII copper levels were significantly higher in the superficial layer of soil throughout the experimental period. It was concluded that copper residues available to plants in soil treated with a mixture containing bovine feces and urine associated with copper sulfate solution and water are higher in the upper layers of the soil showing no significant changes in the deeper layers after consecutive rains.Item Lesões de isquemia arteriovenosa e reperfusão em jejuno de equinos: imunoistoquímica de proteínas de junção e histopatologia(Universidade Federal de Goiás, 2007-12-31) NASCIMENTO, Aline Machado Rapello do; ARAÚJO, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928; BRITO, Luiz Augusto Batista; http://lattes.cnpq.br/3212353728321020To evaluate whether complete arteriovenous ischemia followed by reperfusion of mesenteric blood flow aggravates lesions involving small intestine of horses, β--catenin and occludin expressions were measured in jejune epithelia of seven equines, without definite breed. The animals had been submitted to aseptic ventral midline celiotomy and five jejune segments collected corresponding to a baseline segment, a five minutes segment subjected to ischemia, and three segments collected after being subjected to five, sixty and one hundred and twenty minutes of tecidual reperfusion, respectively. Experimentally induced lesions created by total arteriovenous occlusion followed by reperfusion were similar to naturally acquired lesions, as much in the period of ischemia as in the aggravation of the injuries observed during reperfusion. Changes that occur when ischemic intestine is reperfused are progressive, and include mucosal, submucosal, and serosal edema; polymorphonuclear and mononuclear cell infiltrates; and alterations in vascular patterns. Imunohistochemistry analyses have shown strong staining baseline segments for β-catenin in all animals and its progressive lack of staining during ischemia and reperfusion treatments. That indicates progressive lack of intercellular adherence. Similar results of specific decreases of total expression of Occludin, a tight junction protein, were observed. This protein is involved in regulating the movement of solutes in the paracellular pathway and also plays an important role in the maintenance of cell polarity. The reduction of expressions of both intercellular proteins measured directly demonstrates β-catenin injury due to progression of edema during ischemia and its aggravation during reperfusion and also shows an increase of the permeability of paracelular pathways and its related consequences to degradation of Ocludina.