Efeitos da água e do óleo ozonizados no reparo tecidual de feridas cutâneas experimentalmente induzidas em ratos
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2019-07-09
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Universidade Federal de Goiás
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The relevance of cutaneous wounds in the veterinary routine indicates the importance of the expansion of economically viable therapeutic techniques aimed at its treatment. The application of ozonized solutions, especially ozonated water and oil, are presented as techniques that may contribute in the tissue repair process. This study aimed to evaluate and compare the effects of ozonated water and oil on the tissue repair of experimentally induced wounds in rats. Forty-eight female Wistar albino rats weighing between 250 and 300 grams were used. Single 9 mm in diameter wounds were performed on the dorsum of the animals, between the scapulae. The wounds were treated daily in the different experimental groups, with 12 mice treated with ozonated water (GA), 12 mice with ozonated oil (GO), 12 mice with 0,2% allantoin cream (GAL) and 12 rats treated with 0,9% sodium chloride (GCL). The wounds were submitted to macroscopic morphometry and microscopic evaluation with HE, picrosirius red and immunostaining for vascular endothelial growth factor (VEGF). At the macroscopic evaluation, the GA group animals presented a higher percentage of retraction than the rats of the other groups at days three, eight and 15 after induction, and the GA retraction was statistically larger than that of the rats of the GO and GAL groups on days three and eight (p<0,05). Regarding the type of extracellular matrix (ECM) in the wounds, there was no statistical difference between the studied group. However, picrosirius red staining showed that the GA and GO groups showed higher total collagen deposition at 15 and 22 days of treatment, respectively. Moreover, at these times of evaluation, these groups presented a higher percentage of type I collagen fibers compared to other groups (p <0.05). Moreover, the neovascularization variable was higher in the GO rats wound on all evaluated days, being statistically higher at three and eight days compared to the GCL group, at 15 days compared to the GCL and GAL. At 22 days, the GA group had a significantly smaller number of vessels compared to the other groups, indicating that this group went into scar remodeling more quickly. These findings were corroborated by VEGF immunohistochemistry marking, which was higher in the GO group at all times, which generated significantly greater immunostaining in the animal's wounds at 8 days compared to the GCL group, at 15 days compared to the group GA, and at 22 days for the GA and GCL groups. It is concluded that ozone water and oil at the tested concentrations contribute to the tissue repair process in experimentally induced skin wounds in rats. Ozonized water enhances wound retraction as well as promotes better epithelial organization and accelerates the onset of healing in the maturation and remodeling phase, taking into account the greater deposition of type I collagen at 15 days and less vascularization at 22 days; while ozonized oil at 418,48 mEq/kg of peroxides promotes greater neovascularization during tissue repair, as well as greater deposition of type I collagen from the third week of treatment.
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SANGUANINI, R. C. Efeitos da água e do óleo ozonizados no reparo tecidual de feridas cutâneas experimentalmente induzidas em ratos. 2019. 71 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2019.