Avaliação da condição higiênico-sanitária e pesquisa de Brucella spp. em queijos do tipo Minas frescal comercializados em feiras livres de Goiânia, Brasil
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2020-03-03
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Universidade Federal de Goiás
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Although the occupational disease profile is one of the main epidemiological characteristics of
the Brucella genus the ingestion of contaminated food is an important mean of human
infection, with the cheese being considered the main source of human brucelosis. So, the aim
of the present study was to research the occurrence of Brucella spp. and estimate the
hygienic-sanitary conditions of Minas Frescal cheeses marketed in free markets in Goiânia.
This work was performed from july to november 2019 in Goiânia - GO. Twenty nine street
fairs was visited on North, South, East, West, Southwest, Northwest and Central areas of
Goiânia and fifty cheeses of Minas Fresh type were acquired. The selected samples had
maximum four days of processed. The cheese samples were analysed for pH, titratable
acidity, moisture and alkaline phosphatase activity. To the microbiological tests, realized the
counting to total and thermotolerant coliforms as well as moulds and yeasts. All samples were
analyzed about presence of Brucella spp. by PCR. The values of pH, titratable acidity and
moisture were evaluated by ANOVA, followed by Scott-Knott (p<0,05) test to compair the
means between the seven regions. The datas referents from questionnaires, visual inspection.
alkaline phosphatase activity and microbial counting were descriptively analyzed. The Central,
Northwest and West's cheeses presented lower pH (p<0,05) compared to the other places.
Have been detected in the Central and West's cheeses the most levels of titratable acidity
(p<0,05). The moisture was higher (p<0,05) in cheeses of street fairs from Goiania North and
East regions Just three samples presented negative to the alkaline phosphatase test,
suggesting that three producers making cheese from pasteurized milk. The total coliforms
counting (35ºC ± 1ºC) were higher in the North and Northwest areas and the minimum value
has been seen in Central region. The thermotolerant coliforms counting were higher in North
and Northwest too, being almost the totality of population of coliforms to 35ºC ± 1ºC. Moulds
and yeasts contamination levels were greater in West and Northwest areas than the other
regions. The fifity samples of Minas Fresh cheese were negative for presence of Brucella spp.
DNA. Cheeses of the Minas Frescal type sold in open markets in Goiânia have adequate
standards of pH, acidity and humidity, they are made with raw milk and despite the non
detection of Brucella spp., they have precarious microbiological conditions, which make them
unhealthy for consumption human.
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MENDES, L. A. Avaliação da condição higiênico-sanitária e pesquisa de Brucella spp. em queijos do tipo Minas frescal comercializados em feiras livres de Goiânia, Brasil. 2020. 90 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2020.