Estratégia genômica de vacinologia reversa para identificação de antígenos vacinais de Plasmodium vivax
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2016-05-16
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Universidade Federal de Goiás
Resumo
Malaria is a parasitic disease caused by protozoa of the genus Plasmodium. In 2014
was registered 214 million new cases in worldwide with approximately 480,000
deaths. Brazil is responsible for about half of malaria cases that occur in America,
where the main etiological agent is P. vivax. The absence of effective vaccines against
malaria parasites is a serious obstacle to controlling the disease. In this context, this
study aimed to identify peptides that may be candidates for the development of a
vaccine against P. vivax through a immunoinformatics strategy called the Reverse
Vaccinology (RV). Primarily, we track the P. falciparum proteome in search of
proteins that presented predicted epitopes and were orthologs between species P. vivax
and the species of malaria rodents, P. yoelli. The similarity between proteins and
epitopes of the three species was quantified for excluding those that exhibited low
similarity. Among this proteins, we sought in the literature which had been extensively
studied and / or whether they had been vaccine candidates in previous research. For
proteins that had been little studied or not evaluated, the prediction of B and T
lymphocytes epitopes. Were thus identified 357 proteins of P. falciparum with
predicted epitopes, among which 270 have orthologs in P. vivax and P. yoelli. Of these,
fifty proteins were found to be highly similar between the three species under study,
and 12 had little or no previous study. These 12 proteins were examined to
Immunology Epitope Database program (IEDB) in order to implement the prediction
of epitopes. Through a combinatorial analysis of the different immunoinformatics
prediction methods, 7 proteins were selected as vaccine candidates, based on their
function and / or location, such as export protein (EXP1), proteins expressed on the
surface of the membrane (SERA) or transmembrane domain (MAEBL), or participate
in processes essential for the survival of the parasite (CLAG). These proteins may be
evaluated in the future biological assay constituents such as antigens in a vaccine
against P. vivax parasite.
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Preditores , Malária , Vacina , Plasmodium vivax , Predictors , Malaria , Vaccine , Plasmodium vivax
Citação
TAVARES, T. L. Estratégia genômica de vacinologia reversa para identificação de antígenos vacinais de Plasmodium vivax. 2016. 77 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2016.