Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção
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2019-05-13
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Universidade Federal de Goiás
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Staphylococcus saprophyticus is a gram positive, coagulase negative bacteria and is one
of the most common etiological agent of urinary tract infections (UTI) among sexually
active young females. The infection may be led mainly by sexual intercourse. Little is
known about the molecular features of S. saprophyticus infection model. However,
plasticity in the proteomic profile is detected in the genus Staphylococcus and can be
related with infection ability. In this study, we aimed to analyze the metabolic differences
among three different pathogenic strains of S. saprophyticus (ATCC 15305, 7108 and
9325) using proteomics approaches. The proteomic data revealed variations among the
strains that may lead to different responses in the context of an infection. There are
differences in the carbohydrate metabolism, amino acid metabolism and pathogen defense
against host defenses. Thioredoxin was one of the differentially expressed proteins among
strains, thiol level assays showed similar levels of reduced thiol for ATCC15305 and 9325
strains, in contrast 7108 strain showed considerably low levels of reduced thiol levels
when compared to the other analyzed strains. Urease is one of the main virulence factors
of S. saprophyticus. The cytoplasmatic urease concentrations varied considerably. The
9325 strain had the lowest cytoplasmatic urease activity, however when plated on ureabased agar it showed a high urease activity, which means that probably most of the urease
synthesized is exported to the extracellular milieu. Another important feature is related to
the purine metabolism operon, which is highly regulated on ATCC15305 and exerts an
influence upon the biofilm production in this strain. These results show that, although
belonging to the same species, different S. saprophyticus strains present diverse behavior
in response to different contexts that the microorganism may face in the host.
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SILVA, K. C. S. Análises proteômicas de cepas de Staphylococcus saprophyticus elucidam diferentes estratégias para virulência e infecção. 2019. 101 f. Tese (Doutorado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019.