Imunofenotipagem e avaliação quantitativa de linfócitos circulantes de bovinos da raça curraleiro
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Data
2008-03-03
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Universidade Federal de Goiás
Resumo
The Curraleiro cattle are extremely docile and resistant to infectious diseases
and parasites. They may be used in exploration of low-quality pastures, without
great investments, when other breeds could show a low productivity or even
survival difficulties. This genetic potential runs risk to extinction due to its
replace by more productive breeds. The Curraleiro cattle resistence to illness is
popularly known and many works has been published about it, although its
immunologic system physiology is completely unknown. Such features are
plausible reasons for the preservation of this potentially genetic resource. So,
the mean aim of this study was to establish an immunological profile by marking
and quantification of T and B lymphocytes in Curraleiro breed with
immunocytochemistry. Thus, it was used 116 bovines, male and female, with
different ages from two farms situated at Goiás State, Brazil. The animals were
allotted in groups according to age, sex and origin. Blood samples were
collected and processed in accordance with immunocytochemistry standard
technique using lymphoid markers species-specific, anti-CD3 (MM1A BoCD3)
and anti-LB (LCTB16A clone B-B14), for T and B lymphocytes counting,
respectively. All procedures were accomplished at Veterinary School, Federal
University of Goiás, Brazil. The data were submitted to descriptive statistics and
then to Kruskall Wallis and Mann-Whitney tests. The results showed decreased
levels of leukocytes, lymphocytes, T lymphocytes and B lymphocytes along the
age advance. Absolute values of leukocytes, lymphocytes and T lymphocytes
were higher in males than females. None of the evaluated parameters were
affected by differences of the management carried out at two farms.
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Citação
MORAES, Júlia de Miranda. Immunophenotyping and quantitative assessment of circulating lyphocytes of the breed of Curraleiro Cattle. 2008. 92 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2008.