Estudo da atividade citotóxica, genotóxica e apoptótica de novos complexos de ouro (III) de fluorquinolonas frente às células B16-F10, K562 e A20
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Data
2012-02-28
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Universidade Federal de Goiás
Resumo
The complex of gold K[Au(CN2)], for example, was introduced at the
beginning of the twentieth century for the treatment of tuberculosis and then replaced
for the gold thiolates (I), which were used in the decade of 30 for the treatment of
Rheumatoid Arthritis. The exploration of gold complexes for the use as an anti-cancer
agent, in an attempt to find a drug less toxic that cisplatin. The current study aimed to
evaluate potential cytotoxic and genotoxic, as well as the mechanism of cell death of
three new gold-based compounds, which is bioactive substance of synthetic origin,
using different cell lines in vitro. In the cytotoxicity assay the effects of the three gold
complexes were evaluated in four tumor cell lines (B16-F10, K562 and A20) and in
two normal lines (L-929, MRC-5) through MTT test, the compounds at different
concentrations (0.2, 2, 20, 50, 100, 200 μM) per 48 hours of treatment. The study
was conducted to test electrophoresis on agarose gel at concentrations
corresponding to IC50 of the strains tested. Were also performed cell cycle analysis
and comet test. In statistical analysis for comparison between treated groups and
control them, was used Anova, in a single criterion and Dunnet's Post-test
(GraphPad Prism 3.02). The results obtained with MTT assay the tested strains
MRC-5, L-929, B-16-F10, A20, K562, for the Spar Au compound was (104, 65.1, 45,
48.3, 61 2, respectively) for the Au Levo compound was (52.8, 181, 28.8, 48.9, 50,
respectively) and the Au Nor compound was (65.1, 79.2, 26 6, 24.9, 55, respectively).
After the screening of compounds, more specific tests were performed only with
some strains that showed more promising results. For K562 cells and B16-F10
treated with Au Levo and Au Spar, respectively, observed that the test showed no
degradation of DNA by apoptosis in the tests performed with 48 hours of treatment
with the compounds. The line B16-F10 treated with the compound Au Spar did not
significantly alter cell cycle kinetics and no reported significant damage to DNA after
48 hours of treatment. More specific tests have to be made in order to deepen the
mechanism of cell death, since the tests were not enough to demonstrate how the
compound is acting.
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NUNES, Paula Roberta. Study of cytotoxic, genotoxic and apoptotic complex of new gold (III) of fluoroquinolones against B16-F10 cells, K562 and A20. 2012. 100 f. Dissertação (Mestrado em Ciências da Saúde - Farmácia) - Universidade Federal de Goiás, Goiânia, 2012.