Avaliação reatividade aórtica, função ventricular e suscetibilidade a arritmias cardíacas de ratos submetidos ao modelo de indução de epilepsia pela pilocarpina
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Universidade Federal de Goiás
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The mechanism of sudden death in epilepsy (SUDEP) is not yet elucidated. Cardiac
function impairment is identified as the likely mechanism. Our objective was to
evaluate the cardiovascular function of rats submitted to the pilocarpine model of
epilepsy. Materials and Methods: Wistar rats weighing 250 g at the beginning of the
experiments were randomly divided into two equal groups: The Epilepsy group in
which animals were subjected to an injection of pilocarpine (350mg / kg ip) preceded
(30 minutes) by Methyl scopolamine (1 mg / kg ip) and status epilepticus was
blocked after three hours with diazepam (10 mg / kg ip). Video monitoring (24hs/day)
was performed. The control rats went through exactly the same procedure, but did
not develop spontaneous seizures or status epilepticus. Rats at one month of
epilepsy were sacrificed and had heart and thoracic aorta dissected. Constant
Pressure modified langendorff was performed to evaluate ventricular function and
arrhythmias tests induced by the protocol of ischemia / reperfusion. For evaluation of
the aortic rings reactivity, curves of phenylephrine and acetylcholine (10-10
- 10-6 mol /
L) were performed. At the end of the experiments the hearts were prepared for
histological analysis (picrossírius) and also molecular biology (Kir 6.1 and Kir 6.2).
Results: showed that the hearts of rats with epilepsy had worsened ventricular
function during ischemia compared with the control group, but before and after
ischemia did not find significant differences. The aortic rings of rats with epilepsy are
less responsive to phenylephrine than control rats. Regarding the histological and
molecular biological analyses, our results showed that the hearts of rats with epilepsy
have higher collagen deposition than control groups. Conclusion: Our results point to
significant worsening of cardiac tissue and the ventricular function at ischemia, which
may contribute to SUDEP.
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SILVA, M. Avaliação reatividade aórtica, função ventricular e suscetibilidade a arritmias cardíacas de ratos submetidos ao modelo de indução de epilepsia pela pilocarpina. 2015. 61 f. Dissertação (Mestrado em Ciências Fisiológicas) - Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, 2015.