Determinação de fármacos fenólicos em produtos farmacêuticos utilizando um biossensor de polifenoloxidase, obtida de extrato bruto do fruto da jurubeba (Solanum paniculatum L.)
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2018-03-05
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Universidade Federal de Goiás
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The use of plant enzymes for analytical purposes is increasing every day, especially in the field
of biotechnology linked to the development of new and more specific analytical instruments.
In this way, the development of biosensors has been increasing since these allow the
measurement of the analyte of interest to be performed by the selective transduction of a
parameter of the target-analyte reaction that can be monitored. For this reason, the biological
element that makes up the instrument, becomes an essential component for its construction.
Polyphenoloxidase is an enzyme that catalyzes the conversion of a phenolic compound to a
quinone, which can be reduced electrochemically and thus allows the detection (quantification)
of the analyte of interest. This enzyme is widely distributed in plants and is widely used in the
production of biosensors and the selective evaluation of phenolic drugs in pharmaceutical
formulations and total phenols in industrial effluents. The polyphenoloxidases in this specific
work were extracted from the fruit of Jurubeba (Solanum paniculatum L.) and used in the
development of carbon paste electrochemical biosensors in the determination of phenolic
drugs, such as paracetamol, ascorbic acid, salicylic acid and or methyldopa. Under
experimental conditions the effect of the amount of enzymatic extract in the carbon paste
ranging from 50 to 200 μL and the optimum pH of 3.0 to 9.0 using the differential pulse
voltammetry technique was investigated. After optimization, the intermediate precision of the
biosensor was tested through its reproducibility through the tests of repeatability, time of
conditioning, stability and linearity. Soon after, used in the determination of the drugs,
obtaining a better response in the detection of paracetamol, in this way, the calibration curve
was realized and consequently applied in the determination of paracetamol of commercial
samples in tablets. The biosensor had a linearity in the range of 5 to 245 μM, with a detection
limit of 3 μM. The polyphenoloxidase extracted from the fruit of Jurubeba showed peculiar
characteristics that it provided in the technological development of biosensors with application
in the quality control of phenolic drugs, exhibiting high sensitivity, satisfactory selectivity,
good repeatability and adequate stability.
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ANTUNES, R. S. Determinação de fármacos fenólicos em produtos farmacêuticos utilizando um biossensor de polifenoloxidase, obtida de extrato bruto do fruto da jurubeba (Solanum paniculatum L.). 2018. 72 f. Dissertação (Mestrado em Assistência e Avaliação em Saúde) - Universidade Federal de Goiás, Goiânia, 2018.