Caracterizações biofísicas de lipossomas biomiméticos

Abstract

In this study, we investigated the eects of lipid composition and protein corona formation on the stability and interactions of fusogenic liposomes. The results revealed that incorporating cholesterol into liposomal membranes significantly enhances their stability, making them more resistant to solubilization by TX-100, a widely used detergent. Additionally, the presence of cell membrane fragments promoted preferential interaction with TX-100 molecules, although it reduced their eective solubilization. It was observed that protein corona formation in fusogenic liposomes was more pronounced compared to other types of liposomes, due to the presence of positive charges on their surface. Furthermore, hybrid fusogenic liposomes demonstrated preferential interaction with plasma components, highlighting their potential for biological applications. Fusion process analysis revealed that the presence of PE lipids (phosphatidylethanolamines) increased the dynamics of this process, reducing the energy barrier required for fusion. However, protein corona formation negatively impacted the fusion of liposomes with the various target membranes tested. These findings have significant implications for the development of fusogenic liposome-based drug delivery systems. Understanding how lipid composition and protein interactions influence the stability and fusion properties of these liposomes is crucial for optimizing their performance as controlled drug delivery platforms.