Citotoxicidade de extratos das folhas de gabirobeira (Campomanesia adamantium) em células de osteossarcoma in vitro

Abstract

Osteosarcoma is a highly aggressive malignant bone tumor that has an unfavorable prognosis. The treatment of choice is surgical intervention associated with systemic chemotherapy. However, the chemotherapeutic agents currently employed have several adverse effects on patients that limit their use. Thus, research aimed at new alternatives to the treatment of this cancer is of paramount importance. This study aimed to evaluate, in vitro, the cytotoxic potential of extracts from leaves of gabirobeira in osteosarcoma cells. From the leaves of this plant, the crude ethanolic extract and ethyl acetate were extracted. The cells were cultured and treated with both extracts at different concentrations and at predetermined exposure times. Cell cytotoxicity was analyzed by the Tripan blue exclusion assay and the IC50 calculated by the tetrazolium reduction method. The crude extract presented higher cytotoxicity compared to acetate, and the best result was in the 1.0μL/mL group treated for 48 hours. In addition, the IC50 value for G48 is within the range suggested as the best cytotoxicity. Finally, extracts of the leaves of the gabirobeira presented cytotoxic action in the cells of in vitro osteosarcoma.