Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína
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2015-07-10
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Universidade Federal de Goiás
Resumo
Memora nodosa (Silva Manso) Miers (Bignoniaceae) is a Cerrado plant
popularly known as caroba. In traditional medicine leaves and roots are used by the
wound healing properties and the roots for abdominal pain. From ethanolic root
extract of Memora nodosa (EERMN) was extracted a purinic derivative, allantoin, that
is founded in products for topical use by the wound healing properties. Previous
works revealed anti-inflammatory and antinociceptive properties with EERMN and
allantoin. In this context, we seek evidence of whether, in addition to antiinflammatory
action, there would be some gastric damaging effect. However, after
the initial results obtained, this work sought to evaluate the antiulcerogenic effect of
the extract and the allantoin and elicit the mechanisms involved in this activity. Albino
Swiss mice, weighing 30-35g, maintained on standard conditions of temperature (23
1C) and illumination (12-h light/12-h dark cycle) were used. The gastroprotective
activity was designed in different experimental gastric ulcers models induced by food
restriction, indomethacin, stress, ethanol, ethanol acidity and acetic acid. Seven days
after food restriction the animals treated with EERMN (300 mg/kg p.o.) showed reduction in the index of lesion from 15.01 2.92 to 5.20 1.44. In indomethacin
induced gastric lesion, different EERMN doses (100, 300 and 1000 mg/kg p.o.)
reduced the index of lesion from 12.70 1.70 (control) to 7.20 0.35; 6.37 0.41
and 5.71 0.47 respectively. Seven days after food restriction the animals treated
with EERMN (300 mg/kg p. o.) showed reduction in the lesion index from 15.01
2.92 to 5.20 1.44. When the gastric ulcers were induced by ethanol 75%, the
EERMN reduced the ulcerated area in 69%. The gastric acid secretion parameters
(volume, pH and total acidity) did not alter by the treatment with the plant extract.
However, the treatment with EERMN was capable to avoid the gastric mucus barrier
depletion when compared to control lesion group, although it was not able to alter the
glutathione levels. In the ethanol acidity model, the treatment with allantoin in doses
of 30, 60 and 120 mg/kg reduced ulcerated area from 67.08 3.07% to 51.53 4.67;
42.32 4.93 and 43.18 4.90% respectively. When the lesions were induced by ethanol, allantoin (60 mg/kg p.o.) reduced ulcerated area in 80%. In models of acute
lesions induced by stress and indomethacin, this same allantoin dose reduced the index of lesion from 11.90 0.93 to 6.62 0.49 and from 15.35 0.81 to 7.09 0.96
respectively. In the chronic model, allantoin demonstrated an antiulcerogenic effect
by the reduction of lesion area from 74.41 4.57 to 24.10 0.89 mm2 in the acetic
acid model. In the quantification of gastric mucus, similarly to EERMN, allantoin was
able to prevent the mucus depletion (34.77 4.14) when compared with control
lesion group (19.75 2.53). Moreover, allantoin was able to restore the activity of
catalase enzyme from 403.7 15.1 (control lesion group) to 499.9 16.38 nmol/mg
protein after gastric lesion induced by ethanol. These results demonstrate
antiulcerogenic activity for EERMN and allantoin throughout different mechanisms.
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SILVA, Dayane Moreira da. Avaliação da atividade gastroprotetora do extrato etanólico das raízes da Me-mora nodosa (Silva Manso) Miers (Bignoniaceae) e de seu fitoconstituinte – Alantoína. 2015. 102 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2015.