Análise de celulases e xilanases por fungo isolado a partir do bioma cerrado
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Data
2013-05-06
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Universidade Federal de Goiás
Resumo
Lignocellulosic biomass is constituted by cellulose, hemicellulose and lignin in the
plant cell walls. The conversion of lignocellulose to fermentable sugars mainly depends
on the action of a complex of enzymes that hydrolyze cellulose and hemicellulose. This
study was to analyze the production of cellulases and xylanases by fungi isolated from
soil and decaying material in Cerrado. Initially, the fungi were analyzed for cellulase
production capacity among the fungal cellulase-producing fungus capable of producing
the highest levels of cellulase was selected for the experiments of xylanase and cellulase
production by submerged fermentation and biochemical analysis of the enzyme
secreted. Among the isolated fungi, fungus called IFBMD01 was that higher activity of
cellulase in the supernatant when cultivated with wheat bran as carbon source. This was
identified as Aspergillus cf niger. The enzymes studied with their respective results
were: FPase with better production time of 7 days, showing activity of 0.10 U / mL,
optimum pH 5.5, optimum temperature of 60 ° C; CMCase with better time production
6 days, showing activity of 8.19 U / mL, pH optimum of 5, optimum temperature of 70 º
C; Avicelase with better production time of 9 days, showing activity of 0.01 U / mL, pH
optimum of 5.5 and optimum temperature of 60 ° C; xylanase, with better production
time 3 days, showing activity of 35.5 U / mL, and the optimum pH of 5 and the
optimum temperature of 50 ° C, the β-Glycosidase, with higher production on the sixth
day showing activity of 0.98 U / mL, with the optimum pH 5 and optimum temperature
of 60 ° C. The activity of β-glucosidase showing in the culture supernatant was
influenced by the ions Mn2 +, NH4, K +, Mg2 +, Ca2 +, Ba2 +, Al3 +, Na +, and Co2+, as
well as EDTA, glucose, xylose and cellobiose. In the culture supernatant of the fungus
A. niger IFBMD01 grown on FT for 3 and 6 days protein bands were visualized with
activity against xylan and CMC molecular weight be 30 to be 66 kDa.
Descrição
Palavras-chave
Aspegillus niger , Celulases , Xilanases , Zimograma , Bioma cerradp , Cellulases , Xylanases , Zymogram , Cerrado Biome
Citação
PEREIRA, Douglas Endrigo Perez. Análise de celulases e xilanases por fungo isolado a partir do bioma cerrado. 2013. 101 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2013