Avaliação do efeito da inativação fotodinâmica sobre a Leptospira interrogans e oócitos bovinos
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2017-02-20
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Universidade Federal de Goiás
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Leptospirosis is an important zoonosis and it causes economic losses due to abortion, mastitis,
infertility, and drop in milk production in affected herds. The disease may be disseminated through
urine, water, contaminated soils, venereal and transplacental routes, and there is a risk of transmission
by in vitro embryo manipulation. Thus, with the advancement of reproductive biotechnology, the
concern with the sanity of animals or herds in the face of any disease also transmitted by semen or
embryos has increased. As an alternative for the inactivation of infectious agents, the technique of
photodynamic inactivation (PDI) has became prominent as it does not cause natural microbial
resistance. The method consists in the combination of a photosensitizer (PS), light, and molecular
oxygen, resulting in the formation of reactive oxygen species (ROS) that at high levels induce cell
death. In this study, in order to inactivate in vitro Leptospira interrogans, six PSs were tested at a
concentration of 10 μM and at the irradiation times of 30 and or 60 min. The TMPP and ZnTMPP
porphyrins were efficient, the first one in the 30 min irradiation, the second one in both irradiation
times. Methylene Blue (MB) demonstrated satisfactory inactivation when irradiated for 60 min.
Hematoporphyrin and phthalocyanines did not demonstrate efficiency in the inactivation of leptospires
in the experimental parameters used. When evaluating the action of ZnTMPP and MB on oocyte
viability, both demonstrated to be innocuous to cumulus-oocyte (CCOs) complexes matured in the 30
min, which makes them potential candidates for the disinfection of biological materials or by-products
of animal origin in place of antibiotics, known to cause bacterial resistance.
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BRAZIL, D. S. Avaliação do efeito da inativação fotodinâmica sobre a Leptospira interrogans e oócitos bovinos. 2017. 71 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2017.