Caracterização citogenética de bovinos da raça Nelore (Bos taurus indicus, Linnaeus 1758)
Nenhuma Miniatura disponível
Data
2019-09-13
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
The agricultural sector has been standing out in the Brazilian economy in recent decades for significant
increase in productivity and its growing importance for maintaining the country's balance of trade.
After the introduction of cattle in Brazil and their subsequent dispersal throughout the national
territory, there was an intense process of adaptation of certain groups to specific environmental
conditions of each place or region, which is responsible for the formation of several local Brazilian
cattle breeds. These animals may present numerical and/or structural chromosomal alterations, which
may result in a group of undesired characteristics to the producer that intends to expand his herd or
even for the preservation of characteristics of a species. The aim of this study was to standardize the C,
GTG and NOR banding for the bovine subspecies (Bos taurus indicus, Linnaeus 1758) and
subsequently produce a pancentromeric probe by amplification and labeling the centromeric regions of
autosomal chromosomes of cattle. Bovine blood samples were collected to detect metaphasic
chromosomes using the cell culture method. Subsequently, cytogenetic banding, amplification,
chromosomal probe labeling and FISH technique were performed. Through the banding it was possible
the correct chromosomal pairing of these animals and with the use of the pancentromeric probe, the
marking of the centromere regions of all autosomal chromosomes. These results may offer a new tool
that meets the needs of genetic improvement of these animals.
Descrição
Citação
Amancio, A. P. Caracterização citogenética de bovinos da raça Nelore (Bos taurus indicus, Linnaeus 1758). 2019. 63 f. Tese (Doutorado em Biotecnologia e Biodiversidade em Rede Pró-Centro-Oeste) - Universidade Federal de Goiás, Goiânia, 2019.