Utilização da RMN e ferramentas quimiométricas na avaliação das alterações metabólicas de Citrus sinensis (L.) Osbeck (var. caipira) causadas por Xanthomonas axonopodis pv. citri
Carregando...
Data
2010-06-11
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
The NMR and chemometrics was used and show that is important to
exploratory analysis of monitoring and understanding of the pathogenic
action caused to Xanthomonas axonopodis pv. citri bacteria. In the extracts,
the morphological characteristics were more important in PCA composition
and in HR-MAS analysis, the metabolic variations were more important in
PCA characterization, especially carbohydrates. Thus, there was the
variation especially of carbohydrates, amino acids and organic acids in
regions of lower chemical shift, which AABA can be considered an inherent
compound of plant defensive response. In orange juices, inherent
compounds of fermentation processes have increased their production
demonstrating that the bacterial infection has caused an increase in the rate
of fruit decay. Flavonoids also have showed the variation of their metabolism
and in many cases, there were reduction of their production, which were led
to loss of important compounds for farmacoligic characteristics of orange
leaves. However, important flavonoids like hesperidin and naringenin
remained constant during bacterial infection. HR-MAS data corroborated
those results obtained in solution NMR analysis, indicating that both
techniques are complementary to more comprehensive analysis, since
differences in volatile terpenoids production, for example, were easily
visualized in matrices without pretreatment through HR-MAS NMR. The
biological evaluations have shown that NMR can be a tool in the design of
experiments with the extracts monitoring. The trend of antioxidant activity
data was similar with PCA of extracts, which the correlation between the
results obtained from both techniques confirmed the contribution of amino
acids as important compounds to defensive system of Citrus against the
pathogenic attack.
Descrição
Citação
SILVA, Lorena Mara Alexandre e. Use of NMR and chemometric methods in
evaluation of metabolic changes in Citrus sinensis (L.)
Osbeck (var. redneck) caused by Xanthomonas axonopodis
pv. citri. 2010. 157 f. Dissertação (Mestrado em Educação em Química) - Universidade Federal de Goiás, Goiânia, 2010.