Avaliação da toxicidade aguda oral, atividades antinociceptiva e anti-inflamatória da crotamina isolada da peçonha de Crotalus durissus collilineatus em camundongos
Nenhuma Miniatura disponível
Data
2017-04-28
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Introduction: Crotamine is a low molecular weight cationic polypeptide (4.8 kDa), isolated
from rattlesnake venom. In the previous study the presence of this toxin in the snake venom
Crotalus durissus collilineatus was responsible for anti-edematogenic activity, suggesting its
anti-inflammatory properties. Pain and inflammation are present mechanisms in several
pathologies, being important the search for new compounds candidates for effective drugs
and with less adverse effects than those existing in the market for such treatments.
Objective: To determine acute oral toxicity of crotamine isolated from snake venom C. d.
collilineatus was evaluated and its effects in experimental models of pain and inflammation in
mice was investigated. Methodology: To evaluate acute oral toxicity, the up and down
procedure and hippocratic screening was performed. For the nociceptive and inflammation
assays, acetic acid-induced abdominal writhing, formalin-induced pain, ear edema induced by
croton oil and pleurisy induced by carrageenan were performed. Results and Discussion:
Crotamine did not cause lethality or signs of intoxication in the dose range of 0.34 to 10.88
mg/kg. In the abdominal writhing test, treatments at doses of 0.08, 0.16 and 0.32 mg/kg p.o.
reduced the number of writhings in relation to the control by 34, 57 and 74 %, respectively,
showing a dose-dependent trend. In the formalin-induced pain test it was observed that
crotamine (0.16 mg/kg p.o.) reduced reactivity to pain in the neurogenic phase in 44.8% and
in the inflammatory phase in 59.7%, suggesting antinociceptive activity. In addition,
crotamine (0.16 mg/kg p.o.) was able to reduce ear edema induced by croton oil by 77 %,
showing antidematogenic activity and reduced in 52.3 % the number of total leukocytes in the
pleurisy test, reducing both neutrophil and mononuclear cells migration, thus exhibiting
antimigratory activity. Conclusion: Crotamine did not induce a toxic or lethal effect at the
doses tested. The reductions in writhings, reactivity to pain, ear edema and leukocytes
migration with administration of crotamine were significant, suggesting that it has
antinociceptive and anti-inflammatory activities.
Descrição
Palavras-chave
Citação
MOREIRA, Lorena Alves. Avaliação da toxicidade aguda oral, atividades antinociceptiva e anti-inflamatória da crotamina isolada da peçonha de Crotalus durissus collilineatus em camundongos. 2017. 74 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2017.