Avaliação de polimorfismos nos genes de reparo e detoxificação e a associação com o dano no DNA em etilistas
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2017-07-06
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Universidade Federal de Goiás
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Alcohol abuse is related to approximately 3.3 million annual deaths worldwide and is considered the first risk factor for the global burden of disease in countries of the Americas. Long-term abuse of alcohol induces numerous molecular and biochemical changes in alcohol-related tissues. It is believed that the toxic effects of alcohol are mediated by DNA damage by several mechanisms, such as by the induction of oxidative damage, DNA adducts, crosslinks and DNA strand breaks. In this sense, the aim of this study was to verify the frequency of polymorphisms in the repair and detoxification genes and the association with the DNA damage in alcoholics. The analysis of the obtained results showed a greater genotoxic damage in the alcoholics, when carrying out the Comet Assay. A point of variation was found in the GSTP1 gene and another point of variation in the XRCC1 gene. However, no statistically significant differences were observed between GSTM1 and GSTT1 genotypes and genetic damage. In this context, it can be concluded that the Comet Assay is a very effective and inexpensive method for the analysis of genotoxic damage.
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MELO, C. O. A. Avaliação de polimorfismos nos genes de reparo e detoxificação e a associação com o dano no DNA em etilistas. 2017. 81 f. Tese (Doutorado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2017.