Potencial biotecnológico de linhagens bacterianas endofíticas de Anacardium othonianum Rizzini
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2017-09-29
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Universidade Federal de Goiás
Resumo
Plants are filled with microorganisms that colonize their structures, those capable of
inhabiting plant tissues internally are known as endophytes and provide new nutritional and
defense pathways, acting as a plant strategy to withstand biotic and abiotic adversities.
Anacardium othonianum Rizzini, also known as cerrado-tree-cashew, is one of the main
cashew trees of economic and social importance in the central-western region of Brazil, but
its endophytic bacterial community has not been investigated yet. In this regard, the aim of
this work was to define endophytic bacterial lineages with functional potential to promote
growth of Anacardium othonianum Rizzini. Root fragments of A. othonianum were inoculated
in a culture medium. Grown colonies were isolated, purified and the identification was
performed by comparing the 16S rDNA sequence in the GenBank database. Subsequently, in vitro tests of biostimulation, biofertilization and biocontrol were performed to select lineages with multifactors that promote plant growth. Two endophytic lines were selected and
investigated with in vivo inoculation in A. othonianum to confirm their growth promoting
action. This work is the first report on the isolation, selection and application of endophytic
bacteria that promote plant growth of A. othonianum. Twenty-two species were isolated, of
which Acinetobacter sp. Bac109, Bacillus mycoides Bac160, Lysinibacillus sphaericus Bac161 and Pantoea agglomerans Bac131 with multifactors of interest. Co-inoculation of A.
othonianum with the endophytic bacteria Acinetobacter sp. (Bac109) and Pantoea agglomerans (Bac131), promoted an increase in initial growth.
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FARIA, P. S. A. Potencial biotecnológico de linhagens bacterianas endofíticas de Anacardium othonianum Rizzini. 2017. 81 f. Tese (Doutorado em Biotecnologia e Biodiversidade em Rede Pró-Centro-Oeste) - Universidade Federal de Goiás, Goiânia, 2017.