Estudo químico, quimiotaxonômico e avaliação antiofídica de espécies dos gêneros Copaifera e Marsypianthes
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2022-12-21
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Universidade Federal de Goiás
Resumo
Snakebites have a major impact on public health, especially in isolated communities
without access to prompt medical attention. There are about 100,000 deaths per year
caused by snakebites and three times as many sequelae. The antivenom is the
conventional treatment, but it has no action at the site of the bite in the human body and
is not always within the reach of victims in isolated locations. The use of plants as
treatment in case of snakebites is a reality, because it is an accessible way, with lowcost resource that attenuates the local effects of the bite too. The species Marsypianthes
chamaedrys, known in the northern region as Boia-cáa or Paracari, is widely used in
these cases. In Brazil, besides M. chamaedrys, also occur M. foliolosa, M. montana, M.
burchelli, M. hassleri and M. tubulosa. Except for the first, the other species are still
poorly studied. This work allowed the annotation of metabolites and evaluation of the
anticoagulant and anti-hemolytic action for Marsypianthes species not studied so far.
The anti-hemolytic action of the M. montana extract stands out. In ethnobotanical
studies, the oil extracted from the trunk of copaibas is reported in cases of ophidic
accidents. In this study, we confirmed the anticoagulant and anti-hemolytic action of the
leaf extract of different taxa of Copaifera against Bothrops alternatus venom. The
species Copaifera sp. and C. marginata totally inhibited the coagulant action of the
venom, and the latter showed more than 95% inhibition of hemolytic activity. The
Copaifera genus is composed of over 70 species, of which 16 occur only in Brazil.
There are many gaps in the correct identification of species and varieties of this genus.
Using direct infusion mass spectrometry data associated with chemometric methods
such as PLS-DA, it was possible to identify the main ions responsible for the distinction
of four taxa of Copaifera, including varieties of the same species. Thus, this work
fulfills its objective of providing chemical and biological information about species of
the genera Copaifera and Marsypianthes. Taking advantage of powerful tools such as
the HPLC-MS/MS and the GNPS platform, it was possible to note several metabolites,
among which, many phenolic compounds, which may be associated with the
investigated antiphidic actions.
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TEIXEIRA JÚNIOR, E. F. Estudo químico, quimiotaxonômico e avaliação antiofídica de espécies dos gêneros Copaifera e Marsypianthes. 2022. 208 f. Tese (Doutorado em Química) - Universidade Federal de Goiás, Goiânia, 2022.