Avaliação morfométrica do núcleo de células neoplásicas mamárias malignas de cadelas

Abstract

The study of cell morphology, as well as nuclear measurement, is important in the histopathological evaluation of breast cancer in female dogs, as well as in women. Bearing in mind that one of the parameters for assessing the degree of malignancy of neoplasms is nuclear pleomorphism. An evaluation of nuclear morphometry can be done with the help of bioimaging software, facilitating the analysis of larger numbers of cases, using subjectivity among observers for quantitative values, low and easy to use. Objective: perform nuclear morphometry in malignant breast neoplasms in female dogs and verify the applicability of Feulgen stain in this analysis. Material and methods: for nuclear analysis, photomicrographs of bitches' breast cancer slides, screen capture (400X magnification), use of a microscope with coupling camera (Leica® DMC 2900) and the ImageJ software (NIH Image 1.52d) to display the nuclear parameters: area, perimeter, Feret and circularity. Results: Carcinomas had the highest mean (85.40µm2 ± 0.3159) and the average perimeter (48.33µm2 ± 0.1217), when compared to complex and tubulopapillary carcinoma (P <0.0001). Thus, the largest nuclear measures can be associated with malignant neoplasms with a higher degree of malignancy. Keywords: Breast cancer, feulgen, nuclear morphology.