Análise morfológica e molecular da formação de escleródios do fungo Sclerotinia sclerotiorum

Abstract

Sclerotinia sclerotiorum, is a fungus that causes white mold or white rot, about 400 species of plants. Currently, its incidence has been becoming increasingly harmful to agriculture in various regions of the world. This fungus acquires structures, called sclerotia that guarantee the survival of the soil, for a long period. In the present study, the formation and morphological development of resistance structures was evaluated through light microscopy and scanning electron microscopy. Morphological observations indicate the entire growth process, involve an interweaving of hyphae, in addition to allowing the storage of nutrients and water. In addition to the presence of melanin, which gives protection to the sclerotia. Expressions of genes related to the development of sclerotia were evaluated, suggesting different pathways of DOPA melanin melanization. In addition, sclerodial genes, which are related to the morphology of structures, such as histidine kinase, facts that are discussed throughout the work, are able to improve structures and their mechanisms. Through the filtration of species of Trichoderma spp. (T00 and ALL 42), it was also possible to evaluate the growth inhibition of Sclerotinia sclerotiorum fungus, allowing to infer the forms of control of the phytopathogen.