Caracterização de um Antígeno Rico em Prolina(PRA/Ag2) do fungo patogênico humano Paracoccidioides brasiliensis
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Data
2008-01-31
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Universidade Federal de Goiás
Resumo
The dimorphic fungus Paracoccidioides brasiliensis is the causative agent of the
most frequent systemic mycosis in Latin America. In humans, infection starts by
inhalation of fungal propagules, which reach the pulmonary epithelium and differentiate
into the yeast parasitic phase. Here we describe the characterization of a proline-rich
protein (PRA/Ag2) homologue of P. brasiliensis, a predictable cell wall protein, first
identified in Coccidioides immitis. The protein, the cDNA and genomic sequences were
analyzed. Southern blot analysis suggested that there is one copy of the gene in P.
brasiliensis. The cloned cDNA was expressed in Escherichia coli and the purified
rPbPRA/Ag2 was used to obtain polyclonal antibody. The purified recombinant protein
was recognized by sera of patients with proven paracoccidioidomycosis and not by sera
of healthy individuals. Immunoelectron microscopy and biochemical studies
demonstrated the presence of PbPRA/Ag2 in the fungal cell wall, linked through a GPIanchor.
The expression of the Pbpra/ag2 gene was analyzed by real time PCR and
results demonstrated developmental regulation in phases of P. brasiliensis, with a
higher expression in the mycelium saprobic phase. The protein expression analyses
corroborate the transcript levels.
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CASTRO, Kelly Pacheco de. Characterization of a Proline-Rich Antigen (PRA/Ag2) of the human pathogenic fungus Paracoccidioides brasiliensis. 2008. 85 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2008.