Regulação do metabolismo de ferro em função do pH e caracterização da produção de sideróforos em Staphylococcus saprophyticus
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Data
2018-03-08
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Universidade Federal de Goiás
Resumo
Staphylococcus saprophyticus is a coagulase negative bacteria that is part of the human
microbiota and may also be present in surfaces, food and the environment. It can act as a pathogen
causing urinary tract infections (UTIs) in humans. The ability to capture micro and macro nutrients
is related to the ability to survive and establish infection in pathogenic microorganisms. One of the
micronutrients is iron, which can be acquired by microorganisms through secret siderophores or
iron-reducing system on the cell surface. When S. saprophyticus infection is initiated the bacteria
causes changes in the medium, resulting in a change in the pH of the urine. Iron can be found in
soluble form (Fe 2+) in higher concentration at acid pH, and insoluble (Fe 3+) in higher concentration
at basic pH. In this study, the proteomic profile of S. saprophyticus when grown at acidic and basic
pH was evaluated. The results demonstrate that S. saprophyticus respond to the instances of
modulating the pH of proteins related to iron metabolism. A siderophores production capacity by S.
saprophyticus was also investigated after culturing in minimal SSD medium without iron. The results
demonstrate that this bacteria produces siderophores of the carboxylate type when cultivated in the
absence of iron. Phagocytosis assays in macrophages demonstrate that S. saprophyticus is more
susceptible to death after infection when they are deprived of iron, demonstrating that this element
is important to ensure infection. The present study increased the knowledge of the proteomic and
metabolic flexibility of S. saprophyticus in response to extracellular iron levels.
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Palavras-chave
Proteômica , pH , Sideróforos , Ferro , Infecção urinária , Proteomics , pH , Siderophores , Iron , Urinary infection
Citação
SOUZA, B. S. V. Regulação do metabolismo de ferro em função do pH e caracterização da produção de sideróforos em Staphylococcus saprophyticus. 2018. 52 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2018.