Avaliação das atividades genotóxica, antigenotóxica, angiogênica e potencial de cicatrização do látex da Synadenium umbellatum Pax
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2009-11-27
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Universidade Federal de Goiás
Resumo
Synadenium umbellatum Pax (1894) is a vegetable species from
Euphorbiaceae‟s family. The species of this family produce latex. The latex extracted
from S. umbellatum has been used by the Brazilian people as anti-tumoral, anti-inflammatory and wound healing agents. However, this latex presents toxic
substances and proteolic enzymes. The aim of this work was to evaluate the possible
genotoxic, antigenotoxic, cytotoxic, angiogenic and wound healing activities of S.
umbellatum latex (SuL) in rats. The genotoxic and antigenotoxic activities were
evaluated by mouse bone marrow micronucleus test. An alkylant agent mitomycin C
(4 mg.kg-1 body weight) was used as a positive control. For all doses, micronucleated
polychromatic erythrocytes (MNPCE) frequency was evaluated after 24 hours of
treatment. To evaluate the antimutagenic activity, animals were treated with 10, 30,
50 and 100 mg.kg-1
and 4 mg. kg-1
of mitomycin C (MMC) simultaneously. The
frequency of MNPCE was evaluated 24 hours after exposure. Cytotoxicity was
evaluated by the polychromatic and normochromatic erythrocytes ratio (PCE/NCE).
Angiogenic activity of the SuL was evaluated in chorioallantoic membrane (CAM) of
fertilized chicken eggs. The concentrations of the SuL were of 10 and 20 mg/mL.
Wound healing activity was evaluated in skin of rats. The skin fragment was removed
with area 3 x 2,5 cm and submitted to topic application with 5 mL of SuL
(concentration 10 mg/mL) and positive and negative controls. The results showed
that the SuL was strongly mutagenic, cytotoxic, and antimutagenic, and a moderate
activity of anticytotoxicity. In the evaluation of the angiogenesis, the SuL has
promoted the increase of the proliferation of blood vessels in the chorioallantoic
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membrane of fertilized chicken eggs and it also induced the increase of velocity of
wound healing in rat skin lesion.
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MELO-REIS, P. R. Avaliação das atividades genotóxica, antigenotóxica, angiogênica e potencial de cicatrização do látex da Synadenium umbellatum Pax. 2009. 138 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Goiás, Goiânia, 2009.