Análise proteômica em células leveduriformes do fungo Paracoccidioides sp. em excesso de cobre
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Data
2015-02-24
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Universidade Federal de Goiás
Resumo
Paracoccidioidomycosis is the most prevalent systemic mycosis in Latin America, caused by fungi
of the genus Paracoccidioides. The fungus grows as mycelium in the saprophytic environment,
with the infection caused mycelium propagules and/or conidia, that will differentiate into the
yeast form. Copper is an essential micronutrient for eukaryotes and bacteria, is extremely
important to various proteins, and cofactor of many enzymes and used in many biochemical
processes. However, excessive, copper can cause damage to proteins, lipids and nucleic acids,
inferring the importance of this metal homeostasis. The objective of this study was to evaluate the
fungus Paracoccidioides sp behavior in the presence of copper excess, through proteomics
analysis. In the presence of copper excess, the yeast colonies of Paracoccidioides sp. depicted
brown color. Expression of transcripts encoding CTR3, a copper transporter and the copper
chaperone ATX1 showed inhibition and induction, respectively in the presence of excess copper,
as verified by qRT-PCR. Proteins related to stress responses, with melanin production and
pathways for energy, are regulated, suggesting that Paracoccidioides sp. responds to copper excess
with detoxification strategies, protection and induction of various pathways of obtaining energy.
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Fungo , Metabolismo , Melanina , Virulência , Biologia molecular , Fungi , Metabolismo , Melanin , Virulence , Molecular biology
Citação
PORTIS, I. G. Análise proteômica em células leveduriformes do fungo paracoccidioides sp. em excesso de cobre. 2015. 65 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2015.